Dr. Shalender Bhasin: Optimizing the Diagnosis and Treatment of Hypogonadism 2025

madman

Super Moderator
Nelson's house u heard it here first!

Dr. Shalender Bhasin!

* over the past 4 decades he has become a global leader in the biology of androgens, sarcopenia and aging related functional decline, his research has shaped clinical guidelines, its informed public health policy particularly around testosterone therapy, anabolic interventions and then the development of function promoting therapies




1759884157826.webp





25:40-34:36
Methods for Assessing Free Testosterone (FT) Levels

1759884838598.webp





Implications of the Inter-monomeric Allostery and Dynamic Changes in Binding Affinity of SHBG and Albumin
1759884268372.webp





Huge contribution to the field and has been working behind the scenes with the CDC on a standardized ED procedure/harmonized reference range for free testosterone!

Been waiting on this for years, looking forward to putting an end to this free T s**t show!

Pay attention to 25:40-34:36 of the presentation where he explains the importance of measuring free testosterone using a standardized Equilibrium Dialysis method!

This is what is coming!

Remember what I have been stressing over the years that not all ED devices/procedures are the same!

Interesting that he is still pushing the ensemble allosteric model (EAM) according to Zakharov (cFT-Z) the calculated Tru-T algorithm.

I will be waiting on the new data/validation before jumping the gun here!

This is where it stands as of now!

Bhasin and his team are still active with Phase IIB: Development of TruT Algorithm for Commercializations in Androgen Disorders!

Project end date: 31-August-2026












My reply:

Even then Quest''s ED assay is not the same as the ED assay used in the Jasuja/Bhasin paper that I posted back in 2022 before it was released.

The reference ranges given in that paper only apply to that specific assay (ED device/procedure).

The reference ranges in that paper are not set in stone!

As of now there is no standardized ED procedure/harmonized reference range for free testosterone!

Also need to understand that when it comes to the confusion of the currently used/relied upon ED methods between laboratories for testing free testosterone everyone needs to keep in mind that not all ED devices/procedures are the same.
 
Dr. Shalender Bhasin

* Equilibrium Dialysis is the reference method which I recommend highly, the problem is that the Equilibrium Dialysis method has not been harmonized across laboratories and the reference ranges vary across laboratories so there's a great need for standardizing the procedure and generating population-based harmonized reference ranges and we have done this and published a standardized procedure for Equilibrium Dialysis and published the reference ranges in this paper that you can look up




Partitioning of Circulating Sex Hormones Among Binding Proteins

1759884745711.webp






Methods for Assessing Free Testosterone (FT) Levels
1759885111746.webp





The Binding Isotherms of Testosterone Binding to SHBG Exhibit Multiphasic Profile and Do not Conform to a Single Kd
1759885201546.webp





Apparent Kd of Estradiol's Binding. to SHBG Changes Dynamically with SHBG and E2 Concentrations and Involves Inter-Monomeric Allostery
1759885321309.webp






Implications of the Inter-monomeric Allostery and Dynamic Changes in Binding Affinity of SHBG and Albumin
1759885480743.webp
 

hCG Mixing Calculator

HCG Mixing Protocol Calculator

TRT Hormone Predictor Widget

TRT Hormone Predictor

Predict estradiol, DHT, and free testosterone levels based on total testosterone

⚠️ Medical Disclaimer

This tool provides predictions based on statistical models and should NOT replace professional medical advice. Always consult with your healthcare provider before making any changes to your TRT protocol.

ℹ️ Input Parameters

Normal range: 300-1000 ng/dL

Predicted Hormone Levels

Enter your total testosterone value to see predictions

Results will appear here after calculation

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

Beyond Testosterone Podcast

Online statistics

Members online
2
Guests online
264
Total visitors
266

Latest posts

Back
Top