Question about timing blood test for daily T Propionate + HCG protocol

I’m planning to do some blood test today to check how high my testosterone peaks with daily 20 mg Test Propionate (IM) and daily 150 IU HCG (IM). (After this I will probably switch to testosterone cream).

I’ve read in some places that the peak occurs around 4–5 hours post injection,
but ChatGPT and some other sources say it’s more like 14–16 hours after injection.
So I’m not sure when to go for the lab draw if I want to catch the true peak level.

Also — my HCG ran out yesterday, but I’ll get more later today.
Would it matter for the blood tests if I skip one HCG dose or inject it later in the day?
Or are testosterone changes from HCG more of a slow effect over days rather than something that fluctuates hour-by-hour?

Also for measuring free t, should I just get free t, albumine and shbg or should i measure free t directly?

Thanks in advance — would appreciate any insights or personal data points on timing or protocol tweaks.
 

hCG Mixing Calculator

HCG Mixing Protocol Calculator

TRT Hormone Predictor Widget

TRT Hormone Predictor

Predict estradiol, DHT, and free testosterone levels based on total testosterone

⚠️ Medical Disclaimer

This tool provides predictions based on statistical models and should NOT replace professional medical advice. Always consult with your healthcare provider before making any changes to your TRT protocol.

ℹ️ Input Parameters

Normal range: 300-1000 ng/dL

Predicted Hormone Levels

Enter your total testosterone value to see predictions

Results will appear here after calculation

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

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