Side-by-side Estradiol Test Results

I have these for your consumption where we talk about the differences between LC/MS/MS testing of Estradiol, I thought I'd share the following.

These are the same lab (Quest), same day, same draw
, and look at the difference. Noteworthy is my sensitive test is HIGHER:

Estradiol 46 (< or = 39)
Estradiol Ultrasensitive LC/MS/MS 60 (< or = 29)
 
Very interesting! Weird though as 'conventional wisdom' states that the standard test typically overstates one's E2 levels instead of the other way around.
 
I have these for your consumption where we talk about the differences between LC/MS/MS testing of Estradiol, I thought I'd share the following.

These are the same lab (Quest), same day, same draw
, and look at the difference. Noteworthy is my sensitive test is HIGHER:

Estradiol 46 (< or = 39)
Estradiol Ultrasensitive LC/MS/MS 60 (< or = 29)

If it wasn't "same draw", I would have felt comfortable rationalizing it.
 
Last edited:
The point is the standard default E2 lab is just inaccurate for measuring E2 in men and young children.

The Ultra Sensitive assay is much more accurate and the one you should rely on.

Yes, the default E2 lab tends to over estimate values...but not all the time...it's just not accurate for men and children.

Use the Ultra Sensitive as the correct assay.
 
Interesting to see this post! I've had 2 blood draws several months apart and had the sensitive e2 test run from the same blood draw and both times the difference was statistically insignificant, within a couple of points. I've seen no difference between the tests personally. I'll post the lab results tonight or tomorrow. I realize that technically the sensitive test is more accurate. There's no arguing that. It's shown little value for me, though
 
If it wasn't "same draw", I would have felt comfortable rationalizing it.

It wasn't by design, Defy wrote my script as "Sensitive Estradiol" but checking the Quest Tech's accuracy, I pointed out that she had entered the standard test, so she had to go back and in the process ordered BOTH tests. Made for an interesting comparison. Of course the Ultra is the more accurate test no matter the comparison to the standard test.
 
It wasn't by design, Defy wrote my script as "Sensitive Estradiol" but checking the Quest Tech's accuracy, I pointed out that she had entered the standard test, so she had to go back and in the process ordered BOTH tests. Made for an interesting comparison. Of course the Ultra is the more accurate test no matter the comparison to the standard test.

Are you experiencing any estradiol-related symptoms? High/low...or nothing you're aware of.
 
I have to admit to being irritable and easy to aggitate but I'm kind of a jerkoff anyway so I get them confused. Otherwise a little warm at night, not quite the night sweats.
 

hCG Mixing Calculator

HCG Mixing Protocol Calculator

TRT Hormone Predictor Widget

TRT Hormone Predictor

Predict estradiol, DHT, and free testosterone levels based on total testosterone

⚠️ Medical Disclaimer

This tool provides predictions based on statistical models and should NOT replace professional medical advice. Always consult with your healthcare provider before making any changes to your TRT protocol.

ℹ️ Input Parameters

Normal range: 300-1000 ng/dL

Predicted Hormone Levels

Enter your total testosterone value to see predictions

Results will appear here after calculation

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

Beyond Testosterone Podcast

Online statistics

Members online
3
Guests online
363
Total visitors
366

Latest posts

Back
Top