Quest Diagnostics - Testosterone, Free (Dialysis) and Total, MS

madman

Super Moderator
Just to be clear!


*FT (test code 36170) is calculated by equilibrium dialysis using a modified Vermeulen equation





Quest Diagnostics offers the following measurements:

  • TT (by LC-MS/MS, test code 15983) includes sex-hormone bound globulin (SHBG)-bound T, albumin-bound T, and FT.
  • FT (test code 36170) is calculated by equilibrium dialysis using a modified Vermeulen equation.2
  • Bioavailable T (test code 14966) is TT minus SHBG-bound T. Bioavailable T is calculated using the Sodergard equation.3
 
 
Just to be clear!


*FT (test code 36170) is calculated by equilibrium dialysis using a modified Vermeulen equation





Quest Diagnostics offers the following measurements:

  • TT (by LC-MS/MS, test code 15983) includes sex-hormone bound globulin (SHBG)-bound T, albumin-bound T, and FT.
  • FT (test code 36170) is calculated by equilibrium dialysis using a modified Vermeulen equation.2
  • Bioavailable T (test code 14966) is TT minus SHBG-bound T. Bioavailable T is calculated using the Sodergard equation.3
Is the "modified Vermeulen equation" a more accurate method than what you'd get at Labcorp?
 
Is the "modified Vermeulen equation" a more accurate method than what you'd get at Labcorp?

If you are speaking in terms of ED assays then no.

When it comes to testing free testosterone you need to use the most accurate assays such as the gold standard Equilibrium Dialysis or Equilibrium Ultrafiltration (next best) especially in cases of altered SHBG if you want to know where your FT level truly sits.

Quest as posted above offers the gold standard Equilibrium Dialysis assay.


Labcorp also offers the Equilibrium Dialysis and Ultrafiltration assay.



You can use the Quest or Labcorp ED assay.

Many tend to go with Quests ED assay as it is cheaper especially through Nelson's discounted labs.

 

hCG Mixing Calculator

HCG Mixing Protocol Calculator

TRT Hormone Predictor Widget

TRT Hormone Predictor

Predict estradiol, DHT, and free testosterone levels based on total testosterone

⚠️ Medical Disclaimer

This tool provides predictions based on statistical models and should NOT replace professional medical advice. Always consult with your healthcare provider before making any changes to your TRT protocol.

ℹ️ Input Parameters

Normal range: 300-1000 ng/dL

Predicted Hormone Levels

Enter your total testosterone value to see predictions

Results will appear here after calculation

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

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