Mass spectrometry in the clinical laboratory – What is it about?

madman

Super Moderator
Dr. Hubert Vesper and Brian Keevil, 2 powerhouses in the field here!

Vesper the man behind the standardization/harmonization of free testosterone!

It's coming!




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Mass spectrometry in the clinical laboratory – What is it about?

The aim of this session is to provide a general overview of the principle of mass spectrometry, the different mass spectrometry instruments and different method principles (direct injection, sample preparation prior to analysis, single and multianalyte analysis). Applications related to these different instruments and methodological approaches relevant to laboratory medicine will be provided.




At the end of this session, participants should be able to:
  • Describe the principle of mass spectrometry
  • Understand different methodological approaches used to measure analytes in laboratory medicine
  • Summarize different areas in laboratory medicine where mass spectrometry is being used


Moderator: Hubert Vesper

Presentation Title: Starting with the Basics: How Does Mass Spectrometry Work
Speaker: William (Bill) Clarke

Presentation Title: When and how to make the transition from conventional technology towards mass spectrometry
Speaker: Brian Keevil

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HCG Mixing Protocol Calculator

TRT Hormone Predictor Widget

TRT Hormone Predictor

Predict estradiol, DHT, and free testosterone levels based on total testosterone

⚠️ Medical Disclaimer

This tool provides predictions based on statistical models and should NOT replace professional medical advice. Always consult with your healthcare provider before making any changes to your TRT protocol.

ℹ️ Input Parameters

Normal range: 300-1000 ng/dL

Predicted Hormone Levels

Enter your total testosterone value to see predictions

Results will appear here after calculation

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

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