DragonBits
Well-Known Member
Low Estradiol Concentrations in Men With Subnormal Testosterone Concentrations and Type 2 Diabetes
This is an interesting study of men with T2 diabetes. It's interesting for several reasons outside of diabetes.
1) It directly compares the cheaper immunoassay Vs LC-MS/MS sensitive test for E2 showing how much different the results are.
2) It also concludes that " Free estradiol concentrations were directly related to free testosterone but not to BMI or age."
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Furthermore, BMI was related negatively to testosterone but not to estradiol concentrations.
...
This is important because it has been assumed hitherto that low concentrations of testosterone in obesity are due to an increase in aromatase activity and a parallel increase in estradiol concentrations, which suppress the hypothalamo-hypophysealgonadal axis. It is, however, of interest that FE/FT ratio was significantly related to BMI.
...
Comparison of total estradiol measured by immunoassay and LC-MS/MS Of the subjects, 60 men had total estradiol measured by both the assays. The mean and median total estradiol concentrations measured by immunoassay were 3.95 6 1.31 and 3.9 [3.1–4.8] ng/dL, respectively. In contrast, the mean and median total estradiol concentrations measured by LC-MS/MS were lower by almost half (2.16 6 1.66 and 1.9 [0.8–3.2] ng/dL, respectively; P , 0.001 for comparison with immunoassay). The total estradiol concentrations measured by the two assays correlated only weakly (r = 0.37, P = 0.004)
http://care.diabetesjournals.org/content/diacare/early/2011/06/23/dc11-0208.full.pdf
This is an interesting study of men with T2 diabetes. It's interesting for several reasons outside of diabetes.
1) It directly compares the cheaper immunoassay Vs LC-MS/MS sensitive test for E2 showing how much different the results are.
2) It also concludes that " Free estradiol concentrations were directly related to free testosterone but not to BMI or age."
=========================================================
Furthermore, BMI was related negatively to testosterone but not to estradiol concentrations.
...
This is important because it has been assumed hitherto that low concentrations of testosterone in obesity are due to an increase in aromatase activity and a parallel increase in estradiol concentrations, which suppress the hypothalamo-hypophysealgonadal axis. It is, however, of interest that FE/FT ratio was significantly related to BMI.
...
Comparison of total estradiol measured by immunoassay and LC-MS/MS Of the subjects, 60 men had total estradiol measured by both the assays. The mean and median total estradiol concentrations measured by immunoassay were 3.95 6 1.31 and 3.9 [3.1–4.8] ng/dL, respectively. In contrast, the mean and median total estradiol concentrations measured by LC-MS/MS were lower by almost half (2.16 6 1.66 and 1.9 [0.8–3.2] ng/dL, respectively; P , 0.001 for comparison with immunoassay). The total estradiol concentrations measured by the two assays correlated only weakly (r = 0.37, P = 0.004)
http://care.diabetesjournals.org/content/diacare/early/2011/06/23/dc11-0208.full.pdf
