advia centaur estradiol e2

[Eli1947]

Dear Nelson,
My health provider finly agreed to provid me the information on the estradiol E2 test he uses :
"The ADVIA Centaur Enhanced Estradiol Assay".
The method is used for female and male . in the past he used also another kit called E2 low chiled.
can you coment on that test suitability in male.
my estradiol according to this test went up from ab 30 pmole/l prior to TRT and during gel products to 170 pmole/l with T En ab 70mg/w.
eg from about 8 to 46 ng/ml . I wonder if this is the reason for enhencment in my sexual problem levido and ED?

 

[Nelson Vergel]

I had never heard of it but it seems that it correlates well with the LC-MS/MS one.

Clin Biochem. 2012 Jul;45(10-11):811-5. doi: 10.1016/j.clinbiochem.2012.02.016. Epub 2012 Feb 25.

Performance evaluation of Siemens ADVIA Centaur enhanced estradiol assay and a split sample comparison with liquid chromatography-tandem mass spectrometry.
Chen Y1, Kinney L, Soldin SJ.
Author information
Abstract

OBJECTIVES:
The objective of this study was to evaluate the newly developed Siemens ADVIA Centaur enhanced Estradiol (eE2) assay and compare it with a well-established estradiol liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.

DESIGN AND METHODS:
The Siemens eE2 assay was evaluated using the Clinical and Laboratory Standards Institute evaluation protocols. Split patient samples were compared with the eE2 assay, the current ADVIA Centaur E2-6 Ill assay; and LC-MS/MS method by API5000 mass spectrometer.

RESULTS:
Within-run and total imprecision of the eE2 assay demonstrated coefficient of variations of 5.7%, 3.2%, 1.5%, and 10.4%, 7.3%, and 6.8%, at levels of 380, 752, and 2051 pmol/L, respectively. The method comparisons showed: eE2=0.903(E2-6 III) -16.2, R(2)=0.938, average bias=-12.3%; and eE2=0.946(LC-MS/MS)+19.5, R(2)=0.925, average bias: 0%.

CONCLUSION:
The Siemens eE2 assay correlates well with LC-MS/MS. This method is reliable, and appropriate for routine clinical laboratory use.

 

[Nelson Vergel]

1 pmol/L of estradiol is 0.2724 pg/ml (US units)

We use a cut off (non totally "proven" by research) of 45 pg/ml which translates into 165 pmol/L. Yours is pretty much right there but not excessive enough to freak out. If you feel like treating it, you may want to use no more than 0.5 mg anastrozole per week to start with and retest a month later.

Remember that a low dose of Cialis of 5 to 10 mg per day could be a good erection booster on TRT if you are able to tolerate it. Cialis and other ED drugs have actually been shown to increase nitric oxide needed for boners. They have also shown to decrease cardiovascular risks and prostatic inflammation.

 

[Eli1947]

Thanks Nelson, I have seen the comparison . The Q is whether the method is suitable for the purpose as you alwayes insiste on the sensetive method xxxxx. At this end of the globe the labs do not understand to which method you refer ( may be LC-MS/MS). This is a RIA method.As for your sugestion , Unfortunately I can't use PDE5 inhibitors due to a metabolic interference with a must drug for Leukimia which is the resone for my hypoganadal state.

 

[Nelson Vergel]

Eli1947:

I would have to ask the folks from LabCorp.

I am sorry to hear about your leukemia. So you are getting treatment for it now?

 

[Eli1947]

Thank you for the help and for being there for so many guys from different countries . I just learned that you also had a cancer issue among other issues . I am now 11 years in the business of CML and local prostate cancer. alive and kicking. I am a chemist in my education. I lived in the US when had my post doc at Bell Labs 1980-82 , worked for the pharma RD in active drug development as well as the dosage form development mainly aimed for the us market. Because of the contraindication stated on TRT many years had suffered developed osteoprosis . 3 y ago i manged to lift the ban to be treated with TRT after finding doctors who agreed to treat . On top of that I have issues with conflict of the lekimia drugs with testosterone metabolism and have truble balancing between treatments and side effects.