madman
Super Moderator
[h=2]"The Close Proximity of Sex Hormone Binding Globulin and Estradiol Receptor-beta (ERβ) in PC12 Cells Differentiated with Nerve Growth Factor and Treated with Estradiol"[/b]
1. Abstract
We have demonstrated the production and distribution of Sex Hormone Binding Globulin (SHBG) in the brain. We have also seen that SHBG is internalized into specific brain cells including neurons and that this internalization appears to be linked to the presence of Estradiol Receptor Beta (ERβ). Recently, we demonstrated that differentiating PC12 pheochromocytoma cells into neuron-like cells with Nerve Growth Factor (NGF) and treating them with estradiol elicited expression of both SHBG and ERβ in these cells. This manuscript further confirms that work using two different antibodies for SHBG and ERβ. The purpose of these experiments is to determine whether this co-localization of SHBG and ERβ is of a very close proximity to eventually interact. Next we use confocal microscopy to show that, not only are these two steroid binding proteins found in the same cells, they are found very close to each other near the cell’s plasma membrane. Finally, we use the Duolink[SUP]®[/SUP] technology to demonstrate that SHBG and ERβ are in extremely close proximity (<30 nm) of each other in these cells. Further elucidation of the functional significance of SHBG being in close proximity with ERβ awaits other studies.
https://www.gavinpublishers.com/art...erve-Growth-Factor-and-Treated-with-Estradiol
1. Abstract
We have demonstrated the production and distribution of Sex Hormone Binding Globulin (SHBG) in the brain. We have also seen that SHBG is internalized into specific brain cells including neurons and that this internalization appears to be linked to the presence of Estradiol Receptor Beta (ERβ). Recently, we demonstrated that differentiating PC12 pheochromocytoma cells into neuron-like cells with Nerve Growth Factor (NGF) and treating them with estradiol elicited expression of both SHBG and ERβ in these cells. This manuscript further confirms that work using two different antibodies for SHBG and ERβ. The purpose of these experiments is to determine whether this co-localization of SHBG and ERβ is of a very close proximity to eventually interact. Next we use confocal microscopy to show that, not only are these two steroid binding proteins found in the same cells, they are found very close to each other near the cell’s plasma membrane. Finally, we use the Duolink[SUP]®[/SUP] technology to demonstrate that SHBG and ERβ are in extremely close proximity (<30 nm) of each other in these cells. Further elucidation of the functional significance of SHBG being in close proximity with ERβ awaits other studies.
https://www.gavinpublishers.com/art...erve-Growth-Factor-and-Treated-with-Estradiol
