Vince
Super Moderator
An 82-year-old man presented for opinion and management of recurrent CDI following hospitalization for methicillin-resistant Staphylococcus aureus pneumonia. The patient had previously failed several courses of antibiotics for CDI, including vancomycin, vancomycin with metronidazole, fidaxomicin, and bezlotoxumab, with relapse confirmed via symptom recurrence and positive stool test.
At the time of presentation, the patient was under the care of his primary care physician and his neurologist for the treatment of AD, following a gradual 5-year decline in memory and cognition. The patient was taking memantine (28 mg once daily) and donepezil (23 mg once daily). The patient’s dementia symptoms included confusion, memory loss, depression, and flattened affect. On his most recent Mini-Mental State Examination (MMSE) administered by the neurologist, the patient scored 20, indicating mild cognitive impairment. This result reflected the gastroenterologist’s findings and was within the expected range for patients with AD. The patient’s wife reported that he no longer appeared to enjoy socializing, and required considerable assistance with basic tasks such as food preparation, bathing, and taking his medication. Neuropsychiatric testing revealed significant impairments in the areas of memory and semantic language abilities, nonverbal learning, and divided attention and response inhibition. His scores in these areas were within the first through fifth percentiles for function.
Following a detailed discussion regarding the potential risks and benefits associated with the procedure, the patient underwent a single 300 mL FMT infusion (per the Borody method)15 using stool from the patient’s 85-year-old wife as a donor. The patient’s wife was intellectually acute, with normal affect and stable mood. Following the procedure, the patient’s CDI symptoms resolved, and repeat stool testing 2 months later was negative.
At the follow-up visit 2 months post-FMT, the patient’s wife reported improvements in the patient’s mental acuity and affect. The MMSE was re-administered by the gastroenterologist (and subsequently by the neurologist) and the patient scored 26, indicating normal cognition. Four months post-FMT, the patient reported continued improvement in memory, with no progression in symptoms. The patient now remembered his daughter’s birthday, which he had not been able to recall previously, and was able to correct the physician’s recollections of his symptoms. Six months post-FMT, the patient reported a marked improvement in mood, was more interactive, and showed more expressive affect. Readministration of the MMSE revealed that the patient’s score had further increased to 29.
Because no study was conducted, no ethics approval or consent was required. Verbal consent was obtained from the patient for the publication of the report; however, this was merely a courtesy because all data have been de-identified.
To our knowledge, this is the first report of a case of rapid reversal of AD symptoms in a patient following FMT for recurrent CDI. Improvements in AD symptoms occurred as early as 2 months post-FMT and continued to the 6-month follow-up visit (the date of the last follow-up), with no noted reversion of symptoms. The resolution of symptoms occurred in a stepwise manner: first, there was increased mental acuity and improved affect observed at 2 months post-FMT, and this was followed by marked improvements in memory and mood by 4 and 6 months post-FMT, which were accompanied by more expressive affect. These findings paralleled improvements in the patient’s MMSE scores, which increased to within the range of normal cognition by 2 months post-FMT. Eradication of CDI was also confirmed at the 2-month follow-up visit, via the resolution of symptoms and a negative stool test.
The central role of the gut microbiome in neurological dysfunction has been increasingly recognized, and alterations in gut microbiome composition have consistently been reported in ASD,3 PD,4 and MS.5,6 Rapid and dramatic improvements have also been observed in several of these conditions following manipulation of the gut microbiome, including with FMT, which further supports a causal association. In an open-label study in 18 children with ASD, 2 weeks of vancomycin treatment followed by daily FMT infusions for 7 to 8 weeks resulted in significant improvements in core ASD symptoms, which persisted at the 2-year follow-up.3 Furthermore, in a case series of three patients with MS and underlying gastrointestinal symptoms, 5 to 10 days of FMT infusions resulted in a profound reversal of major neurological symptoms, and led to longstanding disease remission.12 Similarly, a 61-year old female with secondary progressive MS achieved disease stability for over 10 years following FMT for recurrent CDI.13 In addition, in a patient with myoclonus dystonia, significant improvement in tremor and functional deficits were reported following the manipulation of gut microbiota with vancomycin, rifaximin, and metronidazole for chronic diarrhoea.14 In another study, a 71-year old male with longstanding constipation and PD experienced a rapid and dramatic reduction in PD symptoms, including an absence of persistent tremors, glabellar tap reflex, and cogwheel
rigidity, when his constipation was treated with vancomycin, metronidazole, and colchicine.16
Gut bacteria may contribute to the pathogenesis of AD via a number of mechanisms. Microbe-mediated bidirectional communication pathways exist between the gut and the central nervous system, and many of the neurotransmitters that regulate mood and cognition in the brain, such as gamma-aminobutyric acid (GABA) and dopamine, are also synthesized and catabolized by gut bacteria. GABA dysfunction has been shown to play a role in AD,17 and abnormally high GABA concentrations have recently been identified in reactive astrocytes of post-mortem AD patients.18 Molecular mimicry is a well-documented strategy that is commonly employed by pathogens to gain a competitive advantage over their host. It occurs when similarities between microbial proteins and host peptides result in the cross-activation of autoreactive T or B cells and a loss of self-tolerance.19 Rheumatic fever after Streptoccocus pyogenes infection is a classic example of this; antigen cross-reactivity between streptococcal M protein and cardiac myosin results in the targeting of myocardial tissue.19 However, a number of other well-documented molecular mimicry events exist, and a recent database study catalogued 261 validated host–microbial mimicry interactions.20 Although the focus to date has been on the role of molecular mimicry as a cause of autoimmune diseases, this mechanism may also be exploited in the development of AD. The progressive accumulation of β-amyloid protein plaques between neurons represents a hallmark of AD. However, the initiating factor responsible for the development and propagation of these prion-like proteins is unknown. Amyloids are secreted by a wide range of pathogenic and non-pathogenic bacteria, and play an important role in cell adhesion and biofilm formation.10 Similarities between the tertiary structure of microbe-derived amyloids and β-amyloid in AD may trigger antigen cross-reactivity, thus potentially initiating the formation of AD. Interestingly, both microbe-derived amyloids (such as curli) and AD-related β-amyloid are recognized by the same Toll-like receptor (TLR)2/TLR1 complex.21 Finally, dysbiosis itself may contribute to the development of AD. A dysbiosis-induced increase in gut permeability may potentially lead to persistent systemic inflammation, disruption of the blood–brain barrier, and ultimately neurodegeneration.
Neuroinflammation plays a central role in the pathogenesis of AD,22,23 and is characterized by elevated levels of pro-inflammatory cytokines,23 nuclear factor κβ signalling,24 and aggregation of activated microglia in damaged areas.25 The influence of the gut microbiome on neuroinflammation in AD remains poorly understood, as do the exact mechanisms of action of FMT in this disease. However, the potent immunomodulatory effects of FMT are well recognized. FMT has been demonstrated to ameliorate chronic intestinal colitis in both humans and animal models via the downregulation of pro-inflammatory cytokines, promotion of anti-inflammatory responses, and inhibition of nuclear factor κβ activity.26–28 In addition, similarly beneficial effects have since been observed in several other conditions with an inflammatory component.13,29,30 Thus, FMT may have a positive effect on cognitive function in AD via alterations in the levels of circulating cytokines. Alternatively, by restoring the previously impaired intestinal barrier function, FMT may prevent the translocation of neuroactive compounds and metabolites within the central nervous system that regulate mood and cognition and contribute to inflammation. However, further studies are urgently required to elucidate the exact mechanisms by which FMT may ameliorate symptoms in AD.
To our knowledge, this is the first documented case of a rapid reversal of AD symptoms in a patient following FMT for recurrent CDI. Although this is only a preliminary report, the remarkable resolution of AD symptoms following FMT for CDI is instructive and adds to the evidence that suggests a causal association between the gut microbiome and neurological dysfunction. Given the probable role of the gut microbiome in the pathogenesis of AD, modulation of the microbiome represents a promising avenue of treatment. A randomized, double-blind, placebo-controlled trial is currently underway to evaluate the efficacy of oral FMT in AD, the results of which are eagerly anticipated.
At the time of presentation, the patient was under the care of his primary care physician and his neurologist for the treatment of AD, following a gradual 5-year decline in memory and cognition. The patient was taking memantine (28 mg once daily) and donepezil (23 mg once daily). The patient’s dementia symptoms included confusion, memory loss, depression, and flattened affect. On his most recent Mini-Mental State Examination (MMSE) administered by the neurologist, the patient scored 20, indicating mild cognitive impairment. This result reflected the gastroenterologist’s findings and was within the expected range for patients with AD. The patient’s wife reported that he no longer appeared to enjoy socializing, and required considerable assistance with basic tasks such as food preparation, bathing, and taking his medication. Neuropsychiatric testing revealed significant impairments in the areas of memory and semantic language abilities, nonverbal learning, and divided attention and response inhibition. His scores in these areas were within the first through fifth percentiles for function.
Following a detailed discussion regarding the potential risks and benefits associated with the procedure, the patient underwent a single 300 mL FMT infusion (per the Borody method)15 using stool from the patient’s 85-year-old wife as a donor. The patient’s wife was intellectually acute, with normal affect and stable mood. Following the procedure, the patient’s CDI symptoms resolved, and repeat stool testing 2 months later was negative.
At the follow-up visit 2 months post-FMT, the patient’s wife reported improvements in the patient’s mental acuity and affect. The MMSE was re-administered by the gastroenterologist (and subsequently by the neurologist) and the patient scored 26, indicating normal cognition. Four months post-FMT, the patient reported continued improvement in memory, with no progression in symptoms. The patient now remembered his daughter’s birthday, which he had not been able to recall previously, and was able to correct the physician’s recollections of his symptoms. Six months post-FMT, the patient reported a marked improvement in mood, was more interactive, and showed more expressive affect. Readministration of the MMSE revealed that the patient’s score had further increased to 29.
Because no study was conducted, no ethics approval or consent was required. Verbal consent was obtained from the patient for the publication of the report; however, this was merely a courtesy because all data have been de-identified.
To our knowledge, this is the first report of a case of rapid reversal of AD symptoms in a patient following FMT for recurrent CDI. Improvements in AD symptoms occurred as early as 2 months post-FMT and continued to the 6-month follow-up visit (the date of the last follow-up), with no noted reversion of symptoms. The resolution of symptoms occurred in a stepwise manner: first, there was increased mental acuity and improved affect observed at 2 months post-FMT, and this was followed by marked improvements in memory and mood by 4 and 6 months post-FMT, which were accompanied by more expressive affect. These findings paralleled improvements in the patient’s MMSE scores, which increased to within the range of normal cognition by 2 months post-FMT. Eradication of CDI was also confirmed at the 2-month follow-up visit, via the resolution of symptoms and a negative stool test.
The central role of the gut microbiome in neurological dysfunction has been increasingly recognized, and alterations in gut microbiome composition have consistently been reported in ASD,3 PD,4 and MS.5,6 Rapid and dramatic improvements have also been observed in several of these conditions following manipulation of the gut microbiome, including with FMT, which further supports a causal association. In an open-label study in 18 children with ASD, 2 weeks of vancomycin treatment followed by daily FMT infusions for 7 to 8 weeks resulted in significant improvements in core ASD symptoms, which persisted at the 2-year follow-up.3 Furthermore, in a case series of three patients with MS and underlying gastrointestinal symptoms, 5 to 10 days of FMT infusions resulted in a profound reversal of major neurological symptoms, and led to longstanding disease remission.12 Similarly, a 61-year old female with secondary progressive MS achieved disease stability for over 10 years following FMT for recurrent CDI.13 In addition, in a patient with myoclonus dystonia, significant improvement in tremor and functional deficits were reported following the manipulation of gut microbiota with vancomycin, rifaximin, and metronidazole for chronic diarrhoea.14 In another study, a 71-year old male with longstanding constipation and PD experienced a rapid and dramatic reduction in PD symptoms, including an absence of persistent tremors, glabellar tap reflex, and cogwheel
rigidity, when his constipation was treated with vancomycin, metronidazole, and colchicine.16
Gut bacteria may contribute to the pathogenesis of AD via a number of mechanisms. Microbe-mediated bidirectional communication pathways exist between the gut and the central nervous system, and many of the neurotransmitters that regulate mood and cognition in the brain, such as gamma-aminobutyric acid (GABA) and dopamine, are also synthesized and catabolized by gut bacteria. GABA dysfunction has been shown to play a role in AD,17 and abnormally high GABA concentrations have recently been identified in reactive astrocytes of post-mortem AD patients.18 Molecular mimicry is a well-documented strategy that is commonly employed by pathogens to gain a competitive advantage over their host. It occurs when similarities between microbial proteins and host peptides result in the cross-activation of autoreactive T or B cells and a loss of self-tolerance.19 Rheumatic fever after Streptoccocus pyogenes infection is a classic example of this; antigen cross-reactivity between streptococcal M protein and cardiac myosin results in the targeting of myocardial tissue.19 However, a number of other well-documented molecular mimicry events exist, and a recent database study catalogued 261 validated host–microbial mimicry interactions.20 Although the focus to date has been on the role of molecular mimicry as a cause of autoimmune diseases, this mechanism may also be exploited in the development of AD. The progressive accumulation of β-amyloid protein plaques between neurons represents a hallmark of AD. However, the initiating factor responsible for the development and propagation of these prion-like proteins is unknown. Amyloids are secreted by a wide range of pathogenic and non-pathogenic bacteria, and play an important role in cell adhesion and biofilm formation.10 Similarities between the tertiary structure of microbe-derived amyloids and β-amyloid in AD may trigger antigen cross-reactivity, thus potentially initiating the formation of AD. Interestingly, both microbe-derived amyloids (such as curli) and AD-related β-amyloid are recognized by the same Toll-like receptor (TLR)2/TLR1 complex.21 Finally, dysbiosis itself may contribute to the development of AD. A dysbiosis-induced increase in gut permeability may potentially lead to persistent systemic inflammation, disruption of the blood–brain barrier, and ultimately neurodegeneration.
Neuroinflammation plays a central role in the pathogenesis of AD,22,23 and is characterized by elevated levels of pro-inflammatory cytokines,23 nuclear factor κβ signalling,24 and aggregation of activated microglia in damaged areas.25 The influence of the gut microbiome on neuroinflammation in AD remains poorly understood, as do the exact mechanisms of action of FMT in this disease. However, the potent immunomodulatory effects of FMT are well recognized. FMT has been demonstrated to ameliorate chronic intestinal colitis in both humans and animal models via the downregulation of pro-inflammatory cytokines, promotion of anti-inflammatory responses, and inhibition of nuclear factor κβ activity.26–28 In addition, similarly beneficial effects have since been observed in several other conditions with an inflammatory component.13,29,30 Thus, FMT may have a positive effect on cognitive function in AD via alterations in the levels of circulating cytokines. Alternatively, by restoring the previously impaired intestinal barrier function, FMT may prevent the translocation of neuroactive compounds and metabolites within the central nervous system that regulate mood and cognition and contribute to inflammation. However, further studies are urgently required to elucidate the exact mechanisms by which FMT may ameliorate symptoms in AD.
To our knowledge, this is the first documented case of a rapid reversal of AD symptoms in a patient following FMT for recurrent CDI. Although this is only a preliminary report, the remarkable resolution of AD symptoms following FMT for CDI is instructive and adds to the evidence that suggests a causal association between the gut microbiome and neurological dysfunction. Given the probable role of the gut microbiome in the pathogenesis of AD, modulation of the microbiome represents a promising avenue of treatment. A randomized, double-blind, placebo-controlled trial is currently underway to evaluate the efficacy of oral FMT in AD, the results of which are eagerly anticipated.
