Nelson Vergel
Founder, ExcelMale.com
[h=2]Highlights[/b]•Reference ranges for steroid metabolites using LC–MS/MS method
•Negative association between age and DHEA, DHEAS, androstenedione and free testosterone
•Negative association between high body mass index and steroid metabolites
Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC–MS/MS and associations to health markers in 304 men
http://www.sciencedirect.com/science/article/pii/S0009898115301108
[h=2]Abstract[/b]We report reference ranges based on LC–MS/MS for testosterone (T), free testosterone (FT) and its precursors, i.e. 17-hydroxyprogesterone (17-OHP), dehydroepiandrosterone (DHEA), DHEA-sulfate (DHEAS) and androstenedione (Adione), in relation to different health markers and lifestyle factors.
The study was based on 304 healthy men aged 30–61 years participating in a population-based cross-sectional study (Health2008). Examination program consisted of a clinical examination, completion of a self-administered questionnaire and blood sampling. Steroid metabolites were measured by a validated and sensitive LC–MS/MS method.
Older age-groups were significantly associated with decreased concentrations of DHEA, DHEAS, Adione, and FT, while no significant associations with age were shown for 17-OHP or T. Participants with BMI ≥ 30 kg/m[SUP]2[/SUP] had lower age-related steroid metabolite z-scores compared to participants with BMI < 30 kg/m[SUP]2[/SUP], i.e. 17-OHP: − 0.51 vs. 0.08 (p < 0.001); DHEA: − 0.27 vs. 0.09 (p = 0.014); Adione: − 0.29 vs. 0.09 (p = 0.012); T: − 0.99 vs. 0.14 (p < 0.001); and FT − 0.55 vs. 0.05 (p < 0.001), respectively.
In conclusion, this large study on serum steroid metabolites and concomitant assessment of health markers in healthy men provides age-related reference ranges, and furthermore evaluates the impact of lifestyle factors and metabolic syndrome on androgen metabolite levels.
•Negative association between age and DHEA, DHEAS, androstenedione and free testosterone
•Negative association between high body mass index and steroid metabolites
Reference ranges of 17-hydroxyprogesterone, DHEA, DHEAS, androstenedione, total and free testosterone determined by TurboFlow-LC–MS/MS and associations to health markers in 304 men
http://www.sciencedirect.com/science/article/pii/S0009898115301108
[h=2]Abstract[/b]We report reference ranges based on LC–MS/MS for testosterone (T), free testosterone (FT) and its precursors, i.e. 17-hydroxyprogesterone (17-OHP), dehydroepiandrosterone (DHEA), DHEA-sulfate (DHEAS) and androstenedione (Adione), in relation to different health markers and lifestyle factors.
The study was based on 304 healthy men aged 30–61 years participating in a population-based cross-sectional study (Health2008). Examination program consisted of a clinical examination, completion of a self-administered questionnaire and blood sampling. Steroid metabolites were measured by a validated and sensitive LC–MS/MS method.
Older age-groups were significantly associated with decreased concentrations of DHEA, DHEAS, Adione, and FT, while no significant associations with age were shown for 17-OHP or T. Participants with BMI ≥ 30 kg/m[SUP]2[/SUP] had lower age-related steroid metabolite z-scores compared to participants with BMI < 30 kg/m[SUP]2[/SUP], i.e. 17-OHP: − 0.51 vs. 0.08 (p < 0.001); DHEA: − 0.27 vs. 0.09 (p = 0.014); Adione: − 0.29 vs. 0.09 (p = 0.012); T: − 0.99 vs. 0.14 (p < 0.001); and FT − 0.55 vs. 0.05 (p < 0.001), respectively.
In conclusion, this large study on serum steroid metabolites and concomitant assessment of health markers in healthy men provides age-related reference ranges, and furthermore evaluates the impact of lifestyle factors and metabolic syndrome on androgen metabolite levels.
