Mayo Clinic Revolutionizing Men’s Health: Advanced ED-LC-MS/MS & QQQ Technology for Accurate Free Testosterone Measurement

[madman]

No one should be using/elying on the known to be inaccurate direct immunoassay!

Speakers: Jessica Colon-Franco, Ph.D., Cleveland Clinic; Leslie Farris, Ph.D., Cleveland Clinic

Accurate assessment of free testosterone (FT) is essential in evaluating androgen status, particularly in cases where total testosterone may be misleading due to altered binding protein levels. This workshop will describe a practical and state-of-the-art LC-MS/MS method for directly measuring FT from dialysate using equilibrium dialysis (ED), offering a streamlined and efficient solution for clinical laboratories.

Thread 'Optimized state-of-the-art direct equilibrium dialysis for free testosterone analysis by LC-MS/MS: Achieving accurate measurements in hours'

Jul 30, 2025

* Equilibrium dialysis (ED) is the preferred method for measuring FT, followed by ultrafiltration, but these methods are technically challenging and time-consuming. Calculation of FT from total testosterone and SHBG (with or without albumin) has limitations and may be inaccurate. There is a need for reliable, easy-to-use, and high throughput methods for clinical measurement of FT.




Author block

Jessica Colon-Franco, Cleveland Clinic, Cleveland, Ohio; Leslie Farris, Cleveland Clinic, Cleveland, Ohio; Drew Payto, Cleveland Clinic, Cleveland, Ohio; Sarah...

• madman
• hypogonadism; free testosterone; ed assay
• Replies: 3
• Forum: Testosterone and Men's Health Articles

 

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Total testosterone

 

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Thread 'CDC STANDARDIZED TOTAL T and ESTRADIOL ASSAYS and soon to be FREE TESTOSTERONE!'

Jan 5, 2019

How the CDC Clinical Standardization Programs Are Improving Hormone Tests - AACC.org

WHAT PROGRESS HAS CDC MADE WITH STANDARDIZING TOTAL TESTOSTERONE AND ESTRADIOL TESTS?



Since HoSt began in 2010, CDC has had more than 350 participants in 15 countries. Participants have shown measurable improvements for both total testosterone (TT) and estradiol (E2). Specifically, the among-laboratory bias has decreased from 16.5% in 2007 to 2.8% in 2017 for TT and from 54.8% in 2012 to 13.9% in 2017 for E2. Not only has bias improved, but data...

• madman
• Replies: 90
• Forum: Blood Test Discussion

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This needs to be stressed!

 

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