Best day in TRT cycle to get blood drawn for labs?

criggs

New Member
I'm taking weekly injections of cypionate of 100mg, I've usually been getting tested on the morning of the 7th day before in injecting my usual dose of Testosterone. Just want to know if that is the best practice?
 
Yes, measuring a trough like this is pretty typical. The main problem with this approach is that it dampens awareness of how high serum testosterone goes right after injections. A rough estimate for cypionate is that on weekly injections peak serum testosterone could be as high as 2.5 to 3 times higher than the trough value. So you might measure 500 ng/dL and think it's lowish, but at the beginning of the cycle your levels are unnaturally elevated, e.g. 1,250-1,500 ng/dL. With greater awareness guys might be inclined to inject more often, which helps to prevent side effects.
 

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Predict estradiol, DHT, and free testosterone levels based on total testosterone

⚠️ Medical Disclaimer

This tool provides predictions based on statistical models and should NOT replace professional medical advice. Always consult with your healthcare provider before making any changes to your TRT protocol.

ℹ️ Input Parameters

Normal range: 300-1000 ng/dL

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Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

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