UPLC-MS/MS Analysis for Clinical Research of: Free Testosterone and Free T4/Free T3

Simplified Equilibrium Dialysis for the UPLC-MS/MD Analysis of Free Testosterone for Clinical Research

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Do you know where your Free Testosterone level truly sits LOL!

Most GPs, dime. a dozen T-mills let alone some of the more reputable clinics (hint, hint) are still clueless when it comes to testing the most critical blood marker FREE TESTOSTERONE!

No one should be using/relying upon the known to be inaccurate direct immunoassays!

Critical points that need to be hammered home here!




Opportunities for improvement


* Harmonize free testosterone reference ranges across laboratories

* Ensure that only accurate free testosterone assays remain on the market


* Free testosterone should be the predominant laboratory parameter to assesss hypogonadism, not total testosterone

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hCG Mixing Calculator

HCG Mixing Protocol Calculator

TRT Hormone Predictor Widget

TRT Hormone Predictor

Predict estradiol, DHT, and free testosterone levels based on total testosterone

⚠️ Medical Disclaimer

This tool provides predictions based on statistical models and should NOT replace professional medical advice. Always consult with your healthcare provider before making any changes to your TRT protocol.

ℹ️ Input Parameters

Normal range: 300-1000 ng/dL

Predicted Hormone Levels

Enter your total testosterone value to see predictions

Results will appear here after calculation

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

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