Times of Day for 4-Tube Cortisol Test?

eyeheartny

Active Member
What times during the day should I take the saliva samples? I have a kit from Labcorp but theirs is keyed for taking one sample a day, while I want to do 4 samples in one day. I'm not sure when during the day to take each sample.
 
8am
Noon
4pm
Midnight

And it’s best to order this separate from blood labs. Otherwise they won’t take the bloods until your samples are returned.
 
4-Tube Collection (saliva) : When using this method, collect a sample immediately upon waking, 30 minutes after waking, then at noon or evening, and night.

diurnal cortisol in saliva.webp
 

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Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

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