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I never took such a post-injection measurement with longer esters. I'd expect a relatively muted rise on this EOD schedule. I saw little difference between testing immediately pre-injection and testing the day after an injection. With 28 mg T cypionate EOD I measured total serum testosterone of about 1,250 ng/dL. In comparison your 1,400 figure on 40 mg EOD does not seem high; it could reflect individual variation along with lower SHBG.