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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
The Dynamics of Allosteric Binding of Estradiol to SHBG
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<blockquote data-quote="madman" data-source="post: 199560" data-attributes="member: 13851"><p><strong>Figure 6. The two monomers within the SHBG dimer populate distinct conformational clusters, which change dynamically upon ligand binding. Relative frequency distribution of SHBG conformational states occupied by each of the monomers (6A, Monomer 1; 6B monomer 2) in response to the binding of the first and the second estradiol molecule to SHBG dimer. The most parsimonious Markov State modeling analysis shows that six conformational states are involved in this dynamics as depicted in this histogram of the distribution of the two monomers in various conformational clusters in the unliganded, singly-bound, and doubly-bound SHBG. Inset displays the color corresponding to the conformational clusters that each SHBG monomer occupies in the unbound and bound states. As shown in the panels 6A, 6B and 6C, the monomers dynamically repartition predominantly in clusters 1-5 as the SHBG dimer transitions between unliganded, singly-bound, and doubly bound states. We posit that these multi-state conformational equilibria manifest as apparent Kd being sensitive to the SHBG/E2 ratio. For this to be true, one would expect that the conformational arrangement of residues in the ligand-binding pockets in these conformational clusters would be distinct. There we generated pairwise overlays for the clusters occupied in the monomers in unbound, singly- and doubly-bound SHBG dimer. Panels 6C, 6D, and 6E show the spatial distinction in the orientation of ligand binding pocket residues in the various clusters. Figure 6C shows the overlay of LBP residues in clusters 1 (blue) and 2 (green). Figure 6D and 6E illustrate the changes in LBP residues in clusters 3 (orange), 4 (cyan), and 5 (grey), which are predominantly occupied by monomers 1 and 2 in the singly-bound states of SHBG dimer. The color-coding of the residues in panels 6D,6E, and 6F corresponds to the cluster population colors in histograms in figures 6A, 6B, and 6C respectively.</strong></p><p><strong>[ATTACH=full]13804[/ATTACH]</strong></p></blockquote><p></p>
[QUOTE="madman, post: 199560, member: 13851"] [B]Figure 6. The two monomers within the SHBG dimer populate distinct conformational clusters, which change dynamically upon ligand binding. Relative frequency distribution of SHBG conformational states occupied by each of the monomers (6A, Monomer 1; 6B monomer 2) in response to the binding of the first and the second estradiol molecule to SHBG dimer. The most parsimonious Markov State modeling analysis shows that six conformational states are involved in this dynamics as depicted in this histogram of the distribution of the two monomers in various conformational clusters in the unliganded, singly-bound, and doubly-bound SHBG. Inset displays the color corresponding to the conformational clusters that each SHBG monomer occupies in the unbound and bound states. As shown in the panels 6A, 6B and 6C, the monomers dynamically repartition predominantly in clusters 1-5 as the SHBG dimer transitions between unliganded, singly-bound, and doubly bound states. We posit that these multi-state conformational equilibria manifest as apparent Kd being sensitive to the SHBG/E2 ratio. For this to be true, one would expect that the conformational arrangement of residues in the ligand-binding pockets in these conformational clusters would be distinct. There we generated pairwise overlays for the clusters occupied in the monomers in unbound, singly- and doubly-bound SHBG dimer. Panels 6C, 6D, and 6E show the spatial distinction in the orientation of ligand binding pocket residues in the various clusters. Figure 6C shows the overlay of LBP residues in clusters 1 (blue) and 2 (green). Figure 6D and 6E illustrate the changes in LBP residues in clusters 3 (orange), 4 (cyan), and 5 (grey), which are predominantly occupied by monomers 1 and 2 in the singly-bound states of SHBG dimer. The color-coding of the residues in panels 6D,6E, and 6F corresponds to the cluster population colors in histograms in figures 6A, 6B, and 6C respectively. [ATTACH type="full"]13804[/ATTACH][/B] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
The Dynamics of Allosteric Binding of Estradiol to SHBG
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