ExcelMale
Menu
Home
What's new
Latest activity
Forums
New posts
Search forums
What's new
New posts
Latest activity
Videos
Lab Tests
Doctor Finder
Buy Books
About Us
Men’s Health Coaching
Log in
Register
What's new
Search
Search
Search titles only
By:
New posts
Search forums
Menu
Log in
Register
Navigation
Install the app
Install
More options
Contact us
Close Menu
Forums
Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
The Dynamics of Allosteric Binding of Estradiol to SHBG
JavaScript is disabled. For a better experience, please enable JavaScript in your browser before proceeding.
You are using an out of date browser. It may not display this or other websites correctly.
You should upgrade or use an
alternative browser
.
Reply to thread
Message
<blockquote data-quote="madman" data-source="post: 199556" data-attributes="member: 13851"><p><strong>Figure 2. Estradiol binding to SHBG alters the microenvironment of tryptophan residues and quenches the fluorescence emission intensity. Panel A shows the relative distance of tryptophan residues from the estradiol ligand in the binding pocket. The residue coordinates were obtained from the crystal structure (PDB ID: 1LHU). The distances between the alpha carbons are mapped for the five tryptophan residues in SHBG to the C9 position on E2. Panel B shows the steady-state emission spectrum from tryptophan residues as the estradiol concentration was increased from subphysiologic (0.0001 nM) to the supraphysiologic (100 nM) range. The data were collected with 1 mm slit width using lex of 290 nm and emission was collected from 310 nm to 410 nm. Panel C is a plot of the changes in integrated emission from tryptophan residues in 20 nM SHBG at increasing estradiol concentration. The binding curve predicted by the extant, linear model of SHBG: E2 association assuming homogenous interaction with both monomers with a fixed Kd of 2 nM (solid red curve) does not fit the experimental binding data, shown in the solid black symbols (Panel C). Panel D shows plots of the residuals between the predicted (red curve in Figure 2C) and experimentally measured data points at graded E2 concentrations and shows that the prevailing linear binding model exhibits under and overestimation from the experimentally-derived binding curve at various E2 concentrations.</strong></p><p><strong>[ATTACH=full]13800[/ATTACH]</strong></p></blockquote><p></p>
[QUOTE="madman, post: 199556, member: 13851"] [B]Figure 2. Estradiol binding to SHBG alters the microenvironment of tryptophan residues and quenches the fluorescence emission intensity. Panel A shows the relative distance of tryptophan residues from the estradiol ligand in the binding pocket. The residue coordinates were obtained from the crystal structure (PDB ID: 1LHU). The distances between the alpha carbons are mapped for the five tryptophan residues in SHBG to the C9 position on E2. Panel B shows the steady-state emission spectrum from tryptophan residues as the estradiol concentration was increased from subphysiologic (0.0001 nM) to the supraphysiologic (100 nM) range. The data were collected with 1 mm slit width using lex of 290 nm and emission was collected from 310 nm to 410 nm. Panel C is a plot of the changes in integrated emission from tryptophan residues in 20 nM SHBG at increasing estradiol concentration. The binding curve predicted by the extant, linear model of SHBG: E2 association assuming homogenous interaction with both monomers with a fixed Kd of 2 nM (solid red curve) does not fit the experimental binding data, shown in the solid black symbols (Panel C). Panel D shows plots of the residuals between the predicted (red curve in Figure 2C) and experimentally measured data points at graded E2 concentrations and shows that the prevailing linear binding model exhibits under and overestimation from the experimentally-derived binding curve at various E2 concentrations. [ATTACH type="full"]13800[/ATTACH][/B] [/QUOTE]
Insert quotes…
Verification
Post reply
Share this page
Facebook
Twitter
Reddit
Pinterest
Tumblr
WhatsApp
Email
Share
Link
Sponsors
Forums
Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
The Dynamics of Allosteric Binding of Estradiol to SHBG
This site uses cookies to help personalise content, tailor your experience and to keep you logged in if you register.
By continuing to use this site, you are consenting to our use of cookies.
Accept
Learn more…
Top