Along these same lines I was wondering something similar. Does the half life result from the T being slowly absorbed out of the muscle or fat into the blood stream, or due to the body metabolizing the injection to separate the T from the oil ??, or both. I had read that muscle has more blood flow than fat so the injection into muscle will leave the muscle faster due to the blood flow than if injected into fat. As you decrease the time between injections it all seems less relevant because there are multiple injection locations emitting T and the half lives of each injection overlap. I am not noticing any troughs yet with injections E3D.