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Figure 2. Method for linking hydraulic fracturing ingredients (i.e. chemicals) with information on toxicity (ATSDR), gene and protein targets (T3DB), and food additives Everything Added to Food in USA (EAFUS). We linked the hydraulic fracturing ingredients to information on toxicity, gene, and protein targets for each chemical and whether or not the chemical was a food additive using their Chemical Abstracts Service (CAS) numbers. We further linked the gene/proteins to estrogen, testosterone, or hormone pathways using KEGG pathways. 


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