IM Injection Sites - Do They Matter?

Excluding subjective pain/discomfort and risk of injury, are there any pharmacological differences between the three major injection sites? I've read in some places that different sites change the absorption rate and even the bioavailability of the testosterone, but I have never seen this on an authoritative medical/scientific document. All other factors being equal, are there any differences between the delts, glutes, and quads as far as pharmacology is concerned? Assuming there are no differences, would it then be useful to alternate between all 3 sites bilaterally so that you only hit each site once every 6 injections? IE: Left glute, right glute, left quad, right quad, left shoulder, right shoulder, repeat
 
Excluding subjective pain/discomfort and risk of injury, are there any pharmacological differences between the three major injection sites? I've read in some places that different sites change the absorption rate and even the bioavailability of the testosterone, but I have never seen this on an authoritative medical/scientific document. All other factors being equal, are there any differences between the delts, glutes, and quads as far as pharmacology is concerned? Assuming there are no differences, would it then be useful to alternate between all 3 sites bilaterally so that you only hit each site once every 6 injections? IE: Left glute, right glute, left quad, right quad, left shoulder, right shoulder, repeat
No I don't believe that the site matters, I like your rotations injections sties.
 
When I mentioned to Dr. Saya that I had switched from shoulders to quads, and asked if that would make a difference on my blood work, he said it wouldn't.
 
Anywhere that you can easily reach and that does not cause undo pain is fair game for either SubQ or IM injections.
 

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TRT Hormone Predictor

Predict estradiol, DHT, and free testosterone levels based on total testosterone

⚠️ Medical Disclaimer

This tool provides predictions based on statistical models and should NOT replace professional medical advice. Always consult with your healthcare provider before making any changes to your TRT protocol.

ℹ️ Input Parameters

Normal range: 300-1000 ng/dL

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Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

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