That is the current discussion. I don't see any downside to twice a day, and hypothetically speaking, it may be beneficial to do so as it may keep it in your system longer.
I dose 2mg once a day in the AM, mainly for convenience as I already do other injections at night.
The 6 week protocol is what Dr. Seeds recommends in his book, Peptide Protocols. But as we have been discussing here, it seems that the chance of copper toxicity at those doses is pretty small, if nonexistent. So it's up to the individual to determine dosage and frequency with those parameters in mind.