Accuracy and Precision of Total Testosterone (TT) Assays and the Evolution of the TT Reference Range

johann77

New Member
Hi all,

42 yr old biochemist and researcher from Europe. On different forms of TRT for about 4 years.

Made a video about the accuracy and precision of testosterone assays and the evolution of the testosterone reference range. I did so because I have the impression that many men seem to believe (or they are made to believe) that reference ranges are manipulated intentionally for whatever reason. I want to shed light on this belief from the perspective of an analytical scientist.

@Nelson Vergel Not sure if i am allowed to link the video. Just want to bring the science to the people and inform them. Please feel free to delete if it violates forum rules in any way.

 

hCG Mixing Calculator

HCG Mixing Protocol Calculator

TRT Hormone Predictor Widget

TRT Hormone Predictor

Predict estradiol, DHT, and free testosterone levels based on total testosterone

⚠️ Medical Disclaimer

This tool provides predictions based on statistical models and should NOT replace professional medical advice. Always consult with your healthcare provider before making any changes to your TRT protocol.

ℹ️ Input Parameters

Normal range: 300-1000 ng/dL

Predicted Hormone Levels

Enter your total testosterone value to see predictions

Results will appear here after calculation

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038

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