E2 regular assay va sensitive

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wondering if eclia « « regular assay »always overestimates e2 ,every comparaison between the two test I saw went this way,or does eclia sometimes underestimates e2.

since I cannot have sensitive assay I m trying to guess where my e2 could be.probably un impossible mission.the question I m asking myself is how did guys on trt do befor labcorp « invented »the sensitive lc ms assay.
 
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So from my understanding I could use this method to calculate my e2 with eclia
 

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