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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Wildly Different E2 Numbers - LabCorp vs. Quest
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<blockquote data-quote="DragonBits" data-source="post: 126107" data-attributes="member: 18023"><p>technically, both labs could be wrong. One lab could be over estimating E2, one could be underestimating E2.</p><p></p><p>It would have been more clear if you had always used the LC-MS/MS method all the time with both labs.</p><p></p><p>When I read research papers on the subject I get the idea of how complicated it gets.</p><p></p><p>Here is one such paper on two major methods that are widely used to measure E2: indirect and direct immunoassays. In comparison to the gold standard of LC-MS/MS.</p><p></p><p>Comparison of Methods to Measure Low Serum Estradiol Levels in Postmenopausal Women</p><p></p><p><em>RESULTS: Three assays detected E2 in all samples, whereas E2 was detected in only 53% and 72% of samples by 2 other assays. All 5 assays had positive biases, ranging from 6% to 74%, throughout their ranges. CVs were lower with 4 immunoassays than with LC-MS. LC-MS, but none of the direct immunoassays, correlated with serum testosterone and sex steroid–binding globulin.</em></p><p></p><p><em><a href="https://academic.oup.com/jcem/article/91/10/3791/2656357" target="_blank">Comparison of Methods to Measure Low Serum Estradiol Levels in Postmenopausal Women | The Journal of Clinical Endocrinology & Metabolism | Oxford Academic</a></em></p><p></p><p>NOTE that there isn't really a male Vs female E2 test, the real key is expected level of E2.</p><p></p><p>Postmenopausal Women have low levels of E2 similar to men, and experience the same sort of inaccurate measurements using immunoassays.</p><p></p><p>If you goggle the subject, you see a lot of discussion about reasons why immunoassay may not be accurate, both from one time to another in the same lab and between different labs. The discussions become very technical.</p><p></p><p>I wouldn't have used an AI for Quest E2 (ultrasensitive / LC-MS/MS): 54 (range: < 29). Maybe if you had done a Labcorp LC-MS/MS at the same time, it would have shown similar results.</p><p></p><p>I would have waited a 2-4 weeks and tested again. Even if I had some mild symptoms of E2 such as water weight gain.</p><p></p><p>I have used at least 3-4 different lab companies, Labcorp, Quest, ACL and whatever the hospital used, which I don't know what that was. I wasn't my choice, sometimes it's just beyond my control.</p><p></p><p>I actually look more at trends and less at absolute numbers, though I pay attention to both.</p></blockquote><p></p>
[QUOTE="DragonBits, post: 126107, member: 18023"] technically, both labs could be wrong. One lab could be over estimating E2, one could be underestimating E2. It would have been more clear if you had always used the LC-MS/MS method all the time with both labs. When I read research papers on the subject I get the idea of how complicated it gets. Here is one such paper on two major methods that are widely used to measure E2: indirect and direct immunoassays. In comparison to the gold standard of LC-MS/MS. Comparison of Methods to Measure Low Serum Estradiol Levels in Postmenopausal Women [I]RESULTS: Three assays detected E2 in all samples, whereas E2 was detected in only 53% and 72% of samples by 2 other assays. All 5 assays had positive biases, ranging from 6% to 74%, throughout their ranges. CVs were lower with 4 immunoassays than with LC-MS. LC-MS, but none of the direct immunoassays, correlated with serum testosterone and sex steroid–binding globulin.[/I] [I][URL="https://academic.oup.com/jcem/article/91/10/3791/2656357"]Comparison of Methods to Measure Low Serum Estradiol Levels in Postmenopausal Women | The Journal of Clinical Endocrinology & Metabolism | Oxford Academic[/URL][/I] NOTE that there isn't really a male Vs female E2 test, the real key is expected level of E2. Postmenopausal Women have low levels of E2 similar to men, and experience the same sort of inaccurate measurements using immunoassays. If you goggle the subject, you see a lot of discussion about reasons why immunoassay may not be accurate, both from one time to another in the same lab and between different labs. The discussions become very technical. I wouldn't have used an AI for Quest E2 (ultrasensitive / LC-MS/MS): 54 (range: < 29). Maybe if you had done a Labcorp LC-MS/MS at the same time, it would have shown similar results. I would have waited a 2-4 weeks and tested again. Even if I had some mild symptoms of E2 such as water weight gain. I have used at least 3-4 different lab companies, Labcorp, Quest, ACL and whatever the hospital used, which I don't know what that was. I wasn't my choice, sometimes it's just beyond my control. I actually look more at trends and less at absolute numbers, though I pay attention to both. [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Wildly Different E2 Numbers - LabCorp vs. Quest
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