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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Side Effect Management
What is Optimal Estradiol Range for Libido/Erection
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<blockquote data-quote="madman" data-source="post: 171642" data-attributes="member: 13851"><p><strong>Steroid assays history</strong></p><p> <strong></strong></p><p></p><p>In addition to protein hormones, the determination of steroids constitutes the basis of modern endocrinology. <span style="color: rgb(26, 188, 156)"><strong>The use of mass spectrometry for steroids measurement started with analysis of these hormones in urine by GC-MS, that was described for the first time by Horning and Sweeley in 1960 [2].</strong></span> <span style="color: rgb(44, 130, 201)"><strong>Immunoassays have been a significant development in the 1960s. The first steroid measurement by radioimmunoassay (RIA) dates back to 1969 with the determination of estradiol (E2). </strong></span><span style="color: rgb(184, 49, 47)"><strong>Then, RIA has been quickly adapted to other steroids such as testosterone and progesterone. </strong></span><span style="color: rgb(147, 101, 184)"><strong>The sensitivity, accuracy and reproducibility of RIA made that it was the reference method whether for research or clinical practice. In the late 1970s, others immunoassays were developed based on enzymometry, fluorimetry or chemiluminescence. </strong></span>Like RIA, these methods can require pretreatment with a chemical extraction or chromatography, depending on antibody specificity. But, automation made them much faster and simple to use, which contributed to their generalization use in daily practice of clinical laboratories. In recent years, mass spectrometry has experienced major technological advance and has become the method of choice for steroids measurement [3].</p></blockquote><p></p>
[QUOTE="madman, post: 171642, member: 13851"] [B]Steroid assays history [/B] In addition to protein hormones, the determination of steroids constitutes the basis of modern endocrinology. [COLOR=rgb(26, 188, 156)][B]The use of mass spectrometry for steroids measurement started with analysis of these hormones in urine by GC-MS, that was described for the first time by Horning and Sweeley in 1960 [2].[/B][/COLOR] [COLOR=rgb(44, 130, 201)][B]Immunoassays have been a significant development in the 1960s. The first steroid measurement by radioimmunoassay (RIA) dates back to 1969 with the determination of estradiol (E2). [/B][/COLOR][COLOR=rgb(184, 49, 47)][B]Then, RIA has been quickly adapted to other steroids such as testosterone and progesterone. [/B][/COLOR][COLOR=rgb(147, 101, 184)][B]The sensitivity, accuracy and reproducibility of RIA made that it was the reference method whether for research or clinical practice. In the late 1970s, others immunoassays were developed based on enzymometry, fluorimetry or chemiluminescence. [/B][/COLOR]Like RIA, these methods can require pretreatment with a chemical extraction or chromatography, depending on antibody specificity. But, automation made them much faster and simple to use, which contributed to their generalization use in daily practice of clinical laboratories. In recent years, mass spectrometry has experienced major technological advance and has become the method of choice for steroids measurement [3]. [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Side Effect Management
What is Optimal Estradiol Range for Libido/Erection
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