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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
What factors could influence total T result?
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<blockquote data-quote="madman" data-source="post: 219842" data-attributes="member: 13851"><p>You are injecting a whopping dose of 200 mg T/week.</p><p></p><p>1st test was done at the true trough (7 days post-injection/Thur. morning) which had you hitting a very high TT 1361 ng/dL which means that your trough FT is going to be very high even if you have highish/high SHBG your trough FT would most likely be in the mid-high 40 ng/dL.</p><p></p><p>Keep in mind peak TT, FT and estradiol will be much higher.</p><p></p><p>Testing at peak would be much sooner 8-24 hrs post-injection and much sooner than most would think.</p><p></p><p>You then waited roughly 2 days (Saturday morning) before doing your next injection then you had blood work done 6 days 8 hrs later and you were hitting a whopping trough (-16 hrs true trough) TT >1500 ng/dL which should have had your FT absurdly high.</p><p></p><p>Pushing up your TT is going to drive up your FT further.</p><p></p><p>Was blood work done at the same lab using the same assay?</p><p></p><p>Even then sounds like you did not use the most accurate assay for TT (LC-MS/MS) otherwise you would have known where your level sat as the analytical measurement range 1.0 ng/dL - 2,000+ ng/dL.</p><p></p><p></p><p><strong>Method Used for Total Testosterone:</strong></p><p><strong></strong></p><p><strong><em>– Liquid chromatography-tandem mass spectrometry (LC-MS/MS)- No upper or lower limit</em></strong></p><p><strong><em></em></strong></p><p><strong><em>– Analytical sensitivity: 1.0 ng/dL</em></strong></p><p><strong><em></em></strong></p><p><strong><em>– Analytical specificity: no cross-reactivity with other steroid compounds or supplements like biotin/</em></strong></p><p></p><p><em><strong>– Analytical Measurement Range: 1.0 ng/dL to 2,000+ ng/dL</strong></em></p><p></p><p></p><p>Would have been better off comparing labs only if TT/FT were both tested using the same lab/same assays (most accurate).</p><p></p><p>You most likely used the same lab but doubtful the most accurate assays, especially for FT.</p><p></p><p>Would pay no attention to your FT unless you had it tested using the most accurate assays such as Equilibrium Dialysis or Ultrafiltration (next best).</p><p></p><p>Especially in cases of altered SHBG and as you stated yours is high!</p><p></p><p>200 mg/week is a fair amount of T.</p><p></p><p>The downfall of injecting high doses of T once weekly is your peaks will be absurdly high and troughs much lower but even then some are still hitting very high troughs on such protocol (dose T/injection frequency).</p><p></p><p>Let alone blood levels will not be as stable throughout the week which can wreak havoc for some when it comes to energy/mood/erections/libido (ups/downs) due to the rollercoaster ride.</p><p></p><p>If you are one who feels great overall, blood markers are healthy and you are not struggling with any sides then go nuts but you are clearly overmedicated.</p><p></p><p>Would be far better off lowering your dose and injecting twice weekly if your goal is to avoid absurdly high levels let alone at the true trough.</p></blockquote><p></p>
[QUOTE="madman, post: 219842, member: 13851"] You are injecting a whopping dose of 200 mg T/week. 1st test was done at the true trough (7 days post-injection/Thur. morning) which had you hitting a very high TT 1361 ng/dL which means that your trough FT is going to be very high even if you have highish/high SHBG your trough FT would most likely be in the mid-high 40 ng/dL. Keep in mind peak TT, FT and estradiol will be much higher. Testing at peak would be much sooner 8-24 hrs post-injection and much sooner than most would think. You then waited roughly 2 days (Saturday morning) before doing your next injection then you had blood work done 6 days 8 hrs later and you were hitting a whopping trough (-16 hrs true trough) TT >1500 ng/dL which should have had your FT absurdly high. Pushing up your TT is going to drive up your FT further. Was blood work done at the same lab using the same assay? Even then sounds like you did not use the most accurate assay for TT (LC-MS/MS) otherwise you would have known where your level sat as the analytical measurement range 1.0 ng/dL - 2,000+ ng/dL. [B]Method Used for Total Testosterone: [I]– Liquid chromatography-tandem mass spectrometry (LC-MS/MS)- No upper or lower limit – Analytical sensitivity: 1.0 ng/dL – Analytical specificity: no cross-reactivity with other steroid compounds or supplements like biotin/[/I][/B] [I][B]– Analytical Measurement Range: 1.0 ng/dL to 2,000+ ng/dL[/B][/I] Would have been better off comparing labs only if TT/FT were both tested using the same lab/same assays (most accurate). You most likely used the same lab but doubtful the most accurate assays, especially for FT. Would pay no attention to your FT unless you had it tested using the most accurate assays such as Equilibrium Dialysis or Ultrafiltration (next best). Especially in cases of altered SHBG and as you stated yours is high! 200 mg/week is a fair amount of T. The downfall of injecting high doses of T once weekly is your peaks will be absurdly high and troughs much lower but even then some are still hitting very high troughs on such protocol (dose T/injection frequency). Let alone blood levels will not be as stable throughout the week which can wreak havoc for some when it comes to energy/mood/erections/libido (ups/downs) due to the rollercoaster ride. If you are one who feels great overall, blood markers are healthy and you are not struggling with any sides then go nuts but you are clearly overmedicated. Would be far better off lowering your dose and injecting twice weekly if your goal is to avoid absurdly high levels let alone at the true trough. [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
What factors could influence total T result?
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