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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Basics & Questions
Total testosterone MS and Nandrolone
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<blockquote data-quote="tareload" data-source="post: 199232"><p>Short answer, no without separation and sometimes derivatization of the compounds you wouldn't have the selectivity.</p><p></p><p>In the case of LC-MS/MS you would be using chromatography (typically HPLC) to separate the hormones and then Mass SpecX2 to selectively identify and quantify them based on their mass-to-charge ratio and product ion counts (using electron multiplier). Please see the link I shared above on LC-MS/MS and example chromatogram below:</p><p></p><p>[URL unfurl="true"]https://www.sigmaaldrich.com/technical-documents/articles/analytical-applications/hplc/hplc-analysis-of-steroids-g005404.html[/URL]</p><p></p><p>If you are referring to this assay:</p><p>[URL unfurl="true"]https://testdirectory.questdiagnostics.com/test/test-detail/15983/testosterone-total-ms?cc=MASTER[/URL]</p><p></p><p>[ATTACH=full]13695[/ATTACH]</p><p></p><p>It's not just MS but actually chromatography combined with MS. No accredited/commercial lab uses just MS to quantify testosterone. You wouldn't want to use MS by itself since the mass spectra of testosterone and nandrolone (and a bunch of other steroids) are overlapping:</p><p></p><p>[URL unfurl="true"]https://webbook.nist.gov/cgi/cbook.cgi?ID=C58220&Units=SI&Mask=200#Mass-Spec[/URL]</p><p></p><p>[URL unfurl="true"]https://webbook.nist.gov/cgi/cbook.cgi?ID=C434220&Units=SI&Mask=200#Mass-Spec[/URL]</p><p></p><p>Of course the assumption is the lab is using chromatography correctly to properly resolve testosterone from all other sex steroids/hormones in the matrix (see example HPLC column above).</p></blockquote><p></p>
[QUOTE="tareload, post: 199232"] Short answer, no without separation and sometimes derivatization of the compounds you wouldn't have the selectivity. In the case of LC-MS/MS you would be using chromatography (typically HPLC) to separate the hormones and then Mass SpecX2 to selectively identify and quantify them based on their mass-to-charge ratio and product ion counts (using electron multiplier). Please see the link I shared above on LC-MS/MS and example chromatogram below: [URL unfurl="true"]https://www.sigmaaldrich.com/technical-documents/articles/analytical-applications/hplc/hplc-analysis-of-steroids-g005404.html[/URL] If you are referring to this assay: [URL unfurl="true"]https://testdirectory.questdiagnostics.com/test/test-detail/15983/testosterone-total-ms?cc=MASTER[/URL] [ATTACH type="full" alt="1618598107888.png"]13695[/ATTACH] It's not just MS but actually chromatography combined with MS. No accredited/commercial lab uses just MS to quantify testosterone. You wouldn't want to use MS by itself since the mass spectra of testosterone and nandrolone (and a bunch of other steroids) are overlapping: [URL unfurl="true"]https://webbook.nist.gov/cgi/cbook.cgi?ID=C58220&Units=SI&Mask=200#Mass-Spec[/URL] [URL unfurl="true"]https://webbook.nist.gov/cgi/cbook.cgi?ID=C434220&Units=SI&Mask=200#Mass-Spec[/URL] Of course the assumption is the lab is using chromatography correctly to properly resolve testosterone from all other sex steroids/hormones in the matrix (see example HPLC column above). [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Basics & Questions
Total testosterone MS and Nandrolone
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