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Thyroid, Pregnenolone, Progesterone, DHEA, etc
Thyroid, DHEA, Pregnenolone, Progesterone, etc
Thyroid Hormone Promotes Insulin-induced Glucose Uptake by Enhancing Akt Phosphorylation and VAMP2 Translocation in 3T3-L1 Adipocytes
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<blockquote data-quote="Gianluca" data-source="post: 238073" data-attributes="member: 15469"><p><h2>Abstract</h2><p>The purpose of this study was to test a hypothesis that T3 promotes glucose uptake <em>via</em> enhancing insulin-induced Akt phosphorylation and VAMP2 translocation in 3T3-L1 adipocytes. T3 significantly enhanced insulin-induced phosphorylation of Akt, cytoplasma to cell membrane translocations of vesicle-associated membrane protein 2 (VAMP2) and glucose transporter 4 (GLUT4), and glucose uptake in adipocytes. Akt inhibitor X abolished the promoting effects of T3, suggesting that Akt activation is essential for T3 to enhance these insulin-induced events in adipocytes. Knockdown of VAMP2 using siRNA abrogated the effects of T3 on insulin-induced GLUT4 translocation and glucose uptake, suggesting that VAMP2 is an important mediator of these processes. Conclusions - These data suggest that T3 may promote glucose uptake <em>via</em> enhancing insulin-induced phosphorylation of Akt and subsequent translocations of VAMP2 and GLUT4 in 3T3-L1 adipocytes. Akt phosphorylation is necessary for the promoting effects of T3 on insulin-stimulated VAMP2 translocation. Further, VAMP2 is essential for T3 to increase insulin-stimulated translocation of GLUT4 and subsequent uptake of glucose in adipocytes.</p><p><strong>Keywords: </strong>GLUT4, RNAi, T3, Akt, VAMP2</p><p></p><p></p><p></p><p>[URL unfurl="true"]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075354/[/URL]</p></blockquote><p></p>
[QUOTE="Gianluca, post: 238073, member: 15469"] [HEADING=1]Abstract[/HEADING] The purpose of this study was to test a hypothesis that T3 promotes glucose uptake [I]via[/I] enhancing insulin-induced Akt phosphorylation and VAMP2 translocation in 3T3-L1 adipocytes. T3 significantly enhanced insulin-induced phosphorylation of Akt, cytoplasma to cell membrane translocations of vesicle-associated membrane protein 2 (VAMP2) and glucose transporter 4 (GLUT4), and glucose uptake in adipocytes. Akt inhibitor X abolished the promoting effects of T3, suggesting that Akt activation is essential for T3 to enhance these insulin-induced events in adipocytes. Knockdown of VAMP2 using siRNA abrogated the effects of T3 on insulin-induced GLUT4 translocation and glucose uptake, suggesting that VAMP2 is an important mediator of these processes. Conclusions - These data suggest that T3 may promote glucose uptake [I]via[/I] enhancing insulin-induced phosphorylation of Akt and subsequent translocations of VAMP2 and GLUT4 in 3T3-L1 adipocytes. Akt phosphorylation is necessary for the promoting effects of T3 on insulin-stimulated VAMP2 translocation. Further, VAMP2 is essential for T3 to increase insulin-stimulated translocation of GLUT4 and subsequent uptake of glucose in adipocytes. [B]Keywords: [/B]GLUT4, RNAi, T3, Akt, VAMP2 [URL unfurl="true"]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075354/[/URL] [/QUOTE]
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Thyroid, Pregnenolone, Progesterone, DHEA, etc
Thyroid, DHEA, Pregnenolone, Progesterone, etc
Thyroid Hormone Promotes Insulin-induced Glucose Uptake by Enhancing Akt Phosphorylation and VAMP2 Translocation in 3T3-L1 Adipocytes
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