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The ARCHITECT 2nd Generation Total Testosterone assay (CMIA) should be fairly accurate but will struggle when measuring low T concentrations in women/children.

The piss poor FT direct RIA is not accurate!

As I stated FT needs to be tested using the gold standard Equilibrium Dialysis or Ultrafiltration (next best).

Would not rely upon let alone waste my time using the direct immunoassay.

If one does not have access to such assays then you would need to use/rely upon the calculated methods and even then I would be using the newer cFTZ (TruT) especially in cases of altered SHBG.

Unfortunately, you have no clue where your SHBG sits.

Now knowing what assay was used for TT there is a good chance that if your B-vitamin complex had a high amount of biotin then your results could have easily been skewed due to cross-reactivity.

post #24
[URL unfurl="true"]https://www.excelmale.com/forum/threads/testostone-levels-declining-with-injections.22981/page-2#post-197330[/URL]

*In competitive immunoassays, excessive biotin is likely to produce a falsely high result

First off, the number bits:
- TT: 480 (240-870)
- FT: 10 (8-40)
- e2: 15 (11-44)

After 2 months taking a B-vitamin complex:
- TT: 860 (240-870)
- FT: 11 (8-40)
- e2: 15 (11-44)

<blockquote data-quote="madman" data-source="post: 203451" data-attributes="member: 13851">The ARCHITECT 2nd Generation Total Testosterone assay (CMIA) should be fairly accurate but will struggle when measuring low T concentrations in women/children.The piss poor FT direct RIA is not accurate!As I stated FT needs to be tested using the gold standard Equilibrium Dialysis or Ultrafiltration (next best).Would not rely upon let alone waste my time using the direct immunoassay.If one does not have access to such assays then you would need to use/rely upon the calculated methods and even then I would be using the newer cFTZ (TruT) especially in cases of altered SHBG.Unfortunately, you have no clue where your SHBG sits.Now knowing what assay was used for TT there is a good chance that if your B-vitamin complex had a high amount of biotin then your results could have easily been skewed due to cross-reactivity.post #24[URL unfurl=&quot;true&quot;]https://www.excelmale.com/forum/threads/testostone-levels-declining-with-injections.22981/page-2#post-197330[/URL]*In competitive immunoassays, excessive biotin is likely to produce a falsely high resultFirst off, the number bits:- TT: 480 (240-870)- FT: 10 (8-40)- e2: 15 (11-44)After 2 months taking a B-vitamin complex:- TT: 860 (240-870)- FT: 11 (8-40)- e2: 15 (11-44)</blockquote>

[QUOTE="madman, post: 203451, member: 13851"] The ARCHITECT 2nd Generation Total Testosterone assay (CMIA) should be fairly accurate but will struggle when measuring low T concentrations in women/children. The piss poor FT direct RIA is not accurate! As I stated FT needs to be tested using the gold standard Equilibrium Dialysis or Ultrafiltration (next best). Would not rely upon let alone waste my time using the direct immunoassay. If one does not have access to such assays then you would need to use/rely upon the calculated methods and even then I would be using the newer cFTZ (TruT) especially in cases of altered SHBG. Unfortunately, you have no clue where your SHBG sits. Now knowing what assay was used for TT there is a good chance that if your B-vitamin complex had a high amount of biotin then your results could have easily been skewed due to cross-reactivity. [B]post #24[/B] [URL unfurl="true"]https://www.excelmale.com/forum/threads/testostone-levels-declining-with-injections.22981/page-2#post-197330[/URL] [I][B]*In competitive immunoassays, [U]excessive biotin is likely to produce a falsely high result[/U] First off, the number bits: - TT: 480 (240-870) - FT: 10 (8-40) - e2: 15 (11-44) After 2 months taking a [U]B-vitamin complex[/U]: - [U]TT[/U]: [U]860[/U] (240-870) - FT: 11 (8-40)[/B][/I] [B][I]- e2: 15 (11-44)[/I][/B] [/QUOTE]

Verification

TRT Hormone Predictor Widget

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038