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General Health & Fitness
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The Best and Worst Whey Protein Powders
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<blockquote data-quote="Nelson Vergel" data-source="post: 190681" data-attributes="member: 3"><p><strong>Survey of protein-based sport supplements for illegally added anabolic steroids methyltestosterone and 4-androstenedione by UPLC-MS/MS</strong></p><p></p><p></p><p>Steroids</p><p>Available online 5 November 2020, 108758</p><p></p><p>Highlights</p><p>•Simple and rapid method was used to extract methyltestosterone and 4-androstenedione from protein supplement matrices.</p><p></p><p>•LC-MS/MS method with matrix matched calibration was validated for simultaneous quantification of the interested anabolic hormones.</p><p></p><p>•<strong>4-androstenedione was found in 36.67% of whey-based supplements; none of them had methytestosterone.</strong></p><p></p><p></p><p>Abstract</p><p>There is some evidence that marketable supplements contain hormones not declared on the product label. The presence of these androgenic anabolic steroids (AAS) in sports supplements can be considered an adulteration and affect the health of consumers, who are predominantly athletes. This study aimed to measure anabolic hormones (methyltestosterone and 4-androstenedione) in sport supplements. Ultra Performance Liquid chromatography coupled mass spectrometry (UPLC-MS/MS) with electrospray ionization (ESI) in positive mode was employed under the Multiple Reaction Monitoring (MRM) ion program. To overcome matrix effects and quantify the selected analyte, the calibration curve was made using Matrix Match method. The LOQ and LOD were 1 ng/g and 0.3 ng/g for both analytes. The recovery of 4-androstenedione and methyltestosterone was in the range of 86.87-107.35 and 77.31-113.98, respectively. In terms of reproducibility, CV % for 4-androstenedione and methyltestosterone ranged from 6.56-16.87% and 1.45-15.12%, respectively. 4-androstenedione was found in 11 samples including 9 whey as 1.578 ± 0.154 ng/g and 2 whey albumin samples with an amount of 1.134 ng/g and 1.474 ng/g. Consequently, continuous controlling of sport supplements comprising intentionally or unintentionally added androgens could be important for health and discuss in the context of compliance with anti-doping.</p></blockquote><p></p>
[QUOTE="Nelson Vergel, post: 190681, member: 3"] [B]Survey of protein-based sport supplements for illegally added anabolic steroids methyltestosterone and 4-androstenedione by UPLC-MS/MS[/B] Steroids Available online 5 November 2020, 108758 Highlights •Simple and rapid method was used to extract methyltestosterone and 4-androstenedione from protein supplement matrices. •LC-MS/MS method with matrix matched calibration was validated for simultaneous quantification of the interested anabolic hormones. •[B]4-androstenedione was found in 36.67% of whey-based supplements; none of them had methytestosterone.[/B] Abstract There is some evidence that marketable supplements contain hormones not declared on the product label. The presence of these androgenic anabolic steroids (AAS) in sports supplements can be considered an adulteration and affect the health of consumers, who are predominantly athletes. This study aimed to measure anabolic hormones (methyltestosterone and 4-androstenedione) in sport supplements. Ultra Performance Liquid chromatography coupled mass spectrometry (UPLC-MS/MS) with electrospray ionization (ESI) in positive mode was employed under the Multiple Reaction Monitoring (MRM) ion program. To overcome matrix effects and quantify the selected analyte, the calibration curve was made using Matrix Match method. The LOQ and LOD were 1 ng/g and 0.3 ng/g for both analytes. The recovery of 4-androstenedione and methyltestosterone was in the range of 86.87-107.35 and 77.31-113.98, respectively. In terms of reproducibility, CV % for 4-androstenedione and methyltestosterone ranged from 6.56-16.87% and 1.45-15.12%, respectively. 4-androstenedione was found in 11 samples including 9 whey as 1.578 ± 0.154 ng/g and 2 whey albumin samples with an amount of 1.134 ng/g and 1.474 ng/g. Consequently, continuous controlling of sport supplements comprising intentionally or unintentionally added androgens could be important for health and discuss in the context of compliance with anti-doping. [/QUOTE]
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The Best and Worst Whey Protein Powders
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