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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Sex Neurosteroids: hormones made by the brain for the brain
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<blockquote data-quote="madman" data-source="post: 198516" data-attributes="member: 13851"><p><strong>Fig. 3 Estradiol phosphorylates aromatase. (a) Primary embryonic neurons in culture after DIV7 were short-term stimulated with thapsigargin 105 M, letrozole 107 M, or 17b-estradiol 107 M and used for immunoprecipitation. Aromatase was precipitated with a polyclonal antibody to aromatase and subjected to SDS-PAGE. The subsequent Western blot was analyzed with either a monoclonal antibody to aromatase (Acris, SM2222P) or a mixture of a monoclonal antibody to phosphoserine/threonine (PM3801, ECM Biosciences) and a monoclonal antibody to phosphotyrosine (Millipore, 05-321). The level of phosphorylation of immunoprecipitated aromatase after treatment of neurons with thapsigargin is decreased as compared to control levels. In contrast, both letrozole and 17b-estradiol treatment resulted in significantly increased levels of phosphorylation. (Mean% SEM%. p 0.05, n = 3 for thapsigargin, n = 19 for 17b- estradiol, n = 5 for letrozole). (b) Aromatase is phosphorylated in female neurons after 17b-estradiol treatment. Female primary hippocampal neurons in the culture at DIV10 were used for immunoprecipitation and treated either under control conditions or with 17bestradiol (107 M). Aromatase was labeled with 32P-orthophosphate and immunoprecipitated with an antibody directed to aromatase (Acris, SM2222P) and subjected to SDS-PAGE. The autoradiogram of 32P-labeled aromatase is shown above. The band at 54kDa indicates phosphorylated aromatase. Aromatase is more strongly phosphorylated after the application of 17b-estradiol in female neurons. (n=3).</strong></p><p><strong>[ATTACH=full]13542[/ATTACH]</strong></p></blockquote><p></p>
[QUOTE="madman, post: 198516, member: 13851"] [B]Fig. 3 Estradiol phosphorylates aromatase. (a) Primary embryonic neurons in culture after DIV7 were short-term stimulated with thapsigargin 105 M, letrozole 107 M, or 17b-estradiol 107 M and used for immunoprecipitation. Aromatase was precipitated with a polyclonal antibody to aromatase and subjected to SDS-PAGE. The subsequent Western blot was analyzed with either a monoclonal antibody to aromatase (Acris, SM2222P) or a mixture of a monoclonal antibody to phosphoserine/threonine (PM3801, ECM Biosciences) and a monoclonal antibody to phosphotyrosine (Millipore, 05-321). The level of phosphorylation of immunoprecipitated aromatase after treatment of neurons with thapsigargin is decreased as compared to control levels. In contrast, both letrozole and 17b-estradiol treatment resulted in significantly increased levels of phosphorylation. (Mean% SEM%. p 0.05, n = 3 for thapsigargin, n = 19 for 17b- estradiol, n = 5 for letrozole). (b) Aromatase is phosphorylated in female neurons after 17b-estradiol treatment. Female primary hippocampal neurons in the culture at DIV10 were used for immunoprecipitation and treated either under control conditions or with 17bestradiol (107 M). Aromatase was labeled with 32P-orthophosphate and immunoprecipitated with an antibody directed to aromatase (Acris, SM2222P) and subjected to SDS-PAGE. The autoradiogram of 32P-labeled aromatase is shown above. The band at 54kDa indicates phosphorylated aromatase. Aromatase is more strongly phosphorylated after the application of 17b-estradiol in female neurons. (n=3). [ATTACH type="full"]13542[/ATTACH][/B] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Sex Neurosteroids: hormones made by the brain for the brain
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