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General Health & Fitness
Nutrition and Supplements
Sense and nonsense concerning biotin interference in laboratory tests
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<blockquote data-quote="madman" data-source="post: 181187" data-attributes="member: 13851"><p>[URL unfurl="true"]https://academic.oup.com/jes/article/2/6/563/4994589[/URL]</p><p></p><p></p><p><em>*Ten weeks after initial presentation, the patient’s free and total testosterone were measured using liquid chromatography-tandem mass spectroscopy (LC-MS/MS) and found to be within reference intervals (<a href="https://www.excelmale.com/forum/javascript%3A;" target="_blank">Table 1</a>). Health care professionals in the laboratory were consulted regarding the differing measured testosterone concentrations. <span style="color: rgb(184, 49, 47)">A pattern was identified consisting of aberrant hormones by competitive and immunometric immunoassays compared with normal hormone measurements by LC-MS/MS (<a href="https://www.excelmale.com/forum/javascript%3A;" target="_blank">Table 1</a>).</span> <span style="color: rgb(184, 49, 47)"><strong><u>Notably, tests via LC-MS/MS are not prone to biotin interference</u>.</strong></span> Collectively, these findings suggested potential biotin interference with immunoassay testing as a cause for the patient’s aberrant hormonal profile. Ten weeks after presentation, our patient’s sample was thus tested for biotin by LC-MS/MS (research use only), which measured a biotin concentration of 38 ng/mL (<a href="https://www.excelmale.com/forum/javascript%3A;" target="_blank">Table 1</a>).</em></p></blockquote><p></p>
[QUOTE="madman, post: 181187, member: 13851"] [URL unfurl="true"]https://academic.oup.com/jes/article/2/6/563/4994589[/URL] [I]*Ten weeks after initial presentation, the patient’s free and total testosterone were measured using liquid chromatography-tandem mass spectroscopy (LC-MS/MS) and found to be within reference intervals ([URL='https://www.excelmale.com/forum/javascript%3A;']Table 1[/URL]). Health care professionals in the laboratory were consulted regarding the differing measured testosterone concentrations. [COLOR=rgb(184, 49, 47)]A pattern was identified consisting of aberrant hormones by competitive and immunometric immunoassays compared with normal hormone measurements by LC-MS/MS ([URL='https://www.excelmale.com/forum/javascript%3A;']Table 1[/URL]).[/COLOR] [COLOR=rgb(184, 49, 47)][B][U]Notably, tests via LC-MS/MS are not prone to biotin interference[/U].[/B][/COLOR] Collectively, these findings suggested potential biotin interference with immunoassay testing as a cause for the patient’s aberrant hormonal profile. Ten weeks after presentation, our patient’s sample was thus tested for biotin by LC-MS/MS (research use only), which measured a biotin concentration of 38 ng/mL ([URL='https://www.excelmale.com/forum/javascript%3A;']Table 1[/URL]).[/I] [/QUOTE]
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General Health & Fitness
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Sense and nonsense concerning biotin interference in laboratory tests
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