Self Prescribed TRT and AT Home Bloodwork

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Johnny Test

Active Member
I’m on self prescribed TRT (140mg of Testosterone Enanthate per week). Split into two shots Monday and Thursday (70mg each). I use an at home blood test to check my levels every six weeks. Would like to get your input. My blood was taken at the extreme trough (20 minutes BEFORE my Monday shot)

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Defy Medical TRT clinic doctor
I’m on self prescribed TRT (140mg of Testosterone Enanthate per week). Split into two shots Monday and Thursday (70mg each). I use an at home blood test to check my levels every six weeks. Would like to get your input. My blood was taken at the extreme trough (20 minutes BEFORE my Monday shot)

View attachment 13341

140mg T/week (70mg every 3.5 days) labs were done at the trough and you are hitting a TT 785 ng/dL with an SHBG 20.9 nmol/L which would most likely have your FT level on the higher end.

Also, keep in mind that your peak TT/FT level will be higher.

Although TT is important to know FT is what truly matters as it is the active unbound fraction of testosterone responsible for the positive effects.

Relying on the FAI (Free Androgen Index) is pointless and the only way to know where your FT level truly sits on such protocol (dose T/injection frequency) is to have it tested using the most accurate assays such as the gold standard Equilibrium Dialysis or Ultrafiltration.



Methods
Free androgen index

Merits
• Represents the ratio of total
testosterone/SHBG
• Has been shown to correlate with free
testosterone measurements
• Simple to obtain

Problems
• Overly simplistic and inaccurate measure of free testosterone concentrations
• Poor indicator of gonadal status
• Dependent on accurate measurements of total testosterone and SHBG
• Most experts do not favor its use
Screenshot (3845).png





Unfortunately when testing FT many men use/rely upon the direct immunoassay (inaccurate) let alone outdated linear law-of-mass action calculated methods which are flawed.

When getting lab work done it would be wise to use the most accurate assays for TT/estradiol (LC/MS-MS) and FT (Equilibrium Dialysis or Ultrafiltration).

You tested TT, estradiol, and SHBG most likely using the standard immunoassays and unfortunately did not have your FT tested using an accurate assay let alone did not include critical blood markers such as RBCs/hemoglobin/hematocrit.

Sure you can still rely on using the calculated method to get an idea of where your FT sits but the only way to truly know is to have it tested using the most accurate assays (ED or UF).

Not sure why you are getting labs every 6 weeks unless you were constantly tweaking your protocol.

Keep in mind many fail to realize that when starting trt or tweaking a protocol (dose T/injection frequency) that hormones will be in flux during the weeks leading up until blood levels stabilize (4-6 weeks when using TC/TE) and it is common for many during this transition to experience what we call the honeymoon period where there may be a strong increase in libido/erections and overall euphoric feeling due to increasing T levels/dopamine.

Unfortunately, this is temporary and short-lived for most as the body will eventually adjust.

It is also very common for many men to experience ups/downs in energy/mood/libido/erections/recovery during the transition as the body is trying to adjust which can be very misleading.

Even then do understand that once blood levels have stabilized (4-6 weeks) it will take another 2-3 months for the body to fully adapt to those new levels and this is the critical time period when one should gauge how they truly feel overall regarding relief/improvement of low-t symptoms.


When looking at the big picture the first 4-6 weeks is very misleading for most!

2-3 months after blood levels have stabilized if you continue to feel great overall and blood markers remain healthy then one can truly claim that your protocol is effective!

Patience is key.
 
You should look into getting blood work done through Nelsons Discountedlabs.

These panels include the most accurate assays for TT (LC/MS-MS) and FT (Equilibrium Dialysis or Ultrafiltration).

Either would suffice!

1. Testosterone, Total and Free (NO Upper Limit) plus Hematocrit

2. Testosterone, Total, LC/MS and Free (Equilibrium Ultrafiltration)



Estradiol (LC/MS-MS)

 
You should look into getting blood work done through Nelsons Discountedlabs.

These panels include the most accurate assays for TT (LC/MS-MS) and FT (Equilibrium Dialysis or Ultrafiltration).

Either would suffice!

1. Testosterone, Total and Free (NO Upper Limit) plus Hematocrit

2. Testosterone, Total, LC/MS and Free (Equilibrium Ultrafiltration)



Estradiol (LC/MS-MS)

I can’t. I live in the shitbag State of New York or I certainly would.
 
Thanks Madman. I have to work with what I have at the moment. Would you recommend I reduce to 130mg or perhaps 120mg per week? My TT 24 hours after my shot is probably 1200-1400 which would he super physiological.
 
140mg T/week (70mg every 3.5 days) labs were done at the trough and you are hitting a TT 785 ng/dL with an SHBG 20.9 nmol/L which would most likely have your FT level on the higher end.

Also, keep in mind that your peak TT/FT level will be higher.

Although TT is important to know FT is what truly matters as it is the active unbound fraction of testosterone responsible for the positive effects.

Relying on the FAI (Free Androgen Index) is pointless and the only way to know where your FT level truly sits on such protocol (dose T/injection frequency) is to have it tested using the most accurate assays such as the gold standard Equilibrium Dialysis or Ultrafiltration.



Methods
Free androgen index

Merits
• Represents the ratio of total
testosterone/SHBG
• Has been shown to correlate with free
testosterone measurements
• Simple to obtain

Problems
• Overly simplistic and inaccurate measure of free testosterone concentrations
• Poor indicator of gonadal status
• Dependent on accurate measurements of total testosterone and SHBG
• Most experts do not favor its use
View attachment 13342




Unfortunately when testing FT many men use/rely upon the direct immunoassay (inaccurate) let alone outdated linear law-of-mass action calculated methods which are flawed.

When getting lab work done it would be wise to use the most accurate assays for TT/estradiol (LC/MS-MS) and FT (Equilibrium Dialysis or Ultrafiltration).

You tested TT, estradiol, and SHBG most likely using the standard immunoassays and unfortunately did not have your FT tested using an accurate assay let alone did not include critical blood markers such as RBCs/hemoglobin/hematocrit.

Sure you can still rely on using the calculated method to get an idea of where your FT sits but the only way to truly know is to have it tested using the most accurate assays (ED or UF).

Not sure why you are getting labs every 6 weeks unless you were constantly tweaking your protocol.

Keep in mind many fail to realize that when starting trt or tweaking a protocol (dose T/injection frequency) that hormones will be in flux during the weeks leading up until blood levels stabilize (4-6 weeks when using TC/TE) and it is common for many during this transition to experience what we call the honeymoon period where there may be a strong increase in libido/erections and overall euphoric feeling due to increasing T levels/dopamine.

Unfortunately, this is temporary and short-lived for most as the body will eventually adjust.

It is also very common for many men to experience ups/downs in energy/mood/libido/erections/recovery during the transition as the body is trying to adjust which can be very misleading.

Even then do understand that once blood levels have stabilized (4-6 weeks) it will take another 2-3 months for the body to fully adapt to those new levels and this is the critical time period when one should gauge how they truly feel overall regarding relief/improvement of low-t symptoms.


When looking at the big picture the first 4-6 weeks is very misleading for most!

2-3 months after blood levels have stabilized if you continue to feel great overall and blood markers remain healthy then one can truly claim that your protocol is effective!

Patience is key.
Thanks Man. I’ve been on self prescribed TRT for many years. I’ve raised and lowered from 100-140 a week and that’s why I get the home tests. It’s $100 and a simple finger prick. Albeit not as accurate but it’s cheap and easy
 
Thanks Man. I’ve been on self prescribed TRT for many years. I’ve raised and lowered from 100-140 a week and that’s why I get the home tests. It’s $100 and a simple finger prick. Albeit not as accurate but it’s cheap and easy
Feel any differences at different doses between that 100mg-140mg/ week range?
 
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