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I've been on trt for about 5 years and need to get Labs again. Don't have the reference range but it's always with LabCorp. Around 500 total and 21 free, I keep E2 pretty low and my SHBG it's probably around 15. I'm extremely sensitive to estrogen and had gynecomastia surgery many years ago to remove both fat and glandular tissue. My current protocol is 50 mg cypionate twice a week (100 mg total) and 1/8 of a milligram of Anastrozole twice-a-week (1/4 total). I've had many ultrasounds over the last several years and this last ultrasound showed an enlarged liver and enlarged spleen. Liver enzymes have always been within range.