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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Reference Intervals for Free Testosterone in Adult Men Measured Using a Standardized Equilibrium Dialysis Procedure
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<blockquote data-quote="madman" data-source="post: 236912" data-attributes="member: 13851"><p>If it went over anyone's head!</p><p></p><p>Hint (references)!</p><p></p><p></p><p><strong>Jasuja 2022</strong> (<a href="https://onlinelibrary.wiley.com/doi/abs/10.1111/andr.13310" target="_blank">https://onlinelibrary.wiley.com/doi/abs/10.1111/andr.13310</a>)</p><p></p><p>*<em><strong>Equilibrium dialysis was performed in 96-well plates (Harvard Apparatus, Holliston, MA) with semi-permeable membranes that allow species less than 10 kDa to pass through (<u>41,43</u>)</strong></em></p><p></p><p></p><p><strong>41. Zakharov MN, Bhasin S,</strong> Travison TG, Xue R, Ulloor J, Vasan RS, Carter E, Wu F, <strong>Jasuja R.</strong> A multi-step, dynamic allosteric model of testosterone's binding to sex hormone binding globulin. Mol Cell Endocrinol. 2015;399:190-200.</p><p></p><p><strong>43.</strong> <strong>Jasuja R,</strong> Spencer D, Jayaraj A, Peng L, Krishna M, Lawney B, Patel P, Jayaram B, Thayer KM, Beveridge DL,<strong> Bhasin S. </strong>Estradiol induces allosteric coupling and partitioning of sex-hormone-binding globulin monomers among conformational states. iScience. 2021;24(6):102414</p><p></p><p></p><p></p><p><em><strong><em>*Our normative ranges are similar to those reported by pioneers of this field using the legacy method (2) but the <u>percent free testosterone in our reference sample differs from that reported by another laboratory (10,52); this difference in percent free testosterone from that reported by another research laboratory (<2.8%) could be due to differences in buffer composition or in other assay conditions that are not apparent in the published methods</u> <strong>(<u>10,52</u>).</strong> The normative ranges of most commercial laboratories have changed substantially in recent years suggesting changes in their procedures over time (53,54); because procedures used by the commercial laboratories and the details of how reference ranges were derived are not published, an evaluation of these procedures was not feasible.</em></strong></em></p><p></p><p></p><p><strong>10.</strong> <strong>Vermeulen A, </strong>Kaufman JM, Giagulli VA. Influence of some biological indexes on sex hormone-binding globulin and androgen levels in aging or obese males. J Clin Endocrinol Metab. 1996;81(5):1821-1826.</p><p></p><p><strong>52. Fiers T, </strong>Wu F, Moghetti P, Vanderschueren D, Lapauw B, Kaufman JM. Reassessing Free-Testosterone Calculation by Liquid Chromatography-Tandem Mass Spectrometry Direct Equilibrium Dialysis. J Clin Endocrinol Metab. 2018;103(6):2167- 2174</p></blockquote><p></p>
[QUOTE="madman, post: 236912, member: 13851"] If it went over anyone's head! Hint (references)! [B]Jasuja 2022[/B] ([URL]https://onlinelibrary.wiley.com/doi/abs/10.1111/andr.13310[/URL]) *[I][B]Equilibrium dialysis was performed in 96-well plates (Harvard Apparatus, Holliston, MA) with semi-permeable membranes that allow species less than 10 kDa to pass through ([U]41,43[/U])[/B][/I] [B]41. Zakharov MN, Bhasin S,[/B] Travison TG, Xue R, Ulloor J, Vasan RS, Carter E, Wu F, [B]Jasuja R.[/B] A multi-step, dynamic allosteric model of testosterone's binding to sex hormone binding globulin. Mol Cell Endocrinol. 2015;399:190-200. [B]43.[/B] [B]Jasuja R,[/B] Spencer D, Jayaraj A, Peng L, Krishna M, Lawney B, Patel P, Jayaram B, Thayer KM, Beveridge DL,[B] Bhasin S. [/B]Estradiol induces allosteric coupling and partitioning of sex-hormone-binding globulin monomers among conformational states. iScience. 2021;24(6):102414 [I][B][I]*Our normative ranges are similar to those reported by pioneers of this field using the legacy method (2) but the [U]percent free testosterone in our reference sample differs from that reported by another laboratory (10,52); this difference in percent free testosterone from that reported by another research laboratory (<2.8%) could be due to differences in buffer composition or in other assay conditions that are not apparent in the published methods[/U] [B]([U]10,52[/U]).[/B] The normative ranges of most commercial laboratories have changed substantially in recent years suggesting changes in their procedures over time (53,54); because procedures used by the commercial laboratories and the details of how reference ranges were derived are not published, an evaluation of these procedures was not feasible.[/I][/B][/I] [B]10.[/B] [B]Vermeulen A, [/B]Kaufman JM, Giagulli VA. Influence of some biological indexes on sex hormone-binding globulin and androgen levels in aging or obese males. J Clin Endocrinol Metab. 1996;81(5):1821-1826. [B]52. Fiers T, [/B]Wu F, Moghetti P, Vanderschueren D, Lapauw B, Kaufman JM. Reassessing Free-Testosterone Calculation by Liquid Chromatography-Tandem Mass Spectrometry Direct Equilibrium Dialysis. J Clin Endocrinol Metab. 2018;103(6):2167- 2174 [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Reference Intervals for Free Testosterone in Adult Men Measured Using a Standardized Equilibrium Dialysis Procedure
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