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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Reference Intervals for Free Testosterone in Adult Men Measured Using a Standardized Equilibrium Dialysis Procedure
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<blockquote data-quote="madman" data-source="post: 236368" data-attributes="member: 13851"><p><em><strong>*Most of the circulating testosterone is bound to sex hormone-binding globulin (SHBG) and human serum albumin (HSA), and a smaller fraction is bound to cortisol-binding globulin and orosomucoid; <u>only less than 4.0% of circulating testosterone is unbound or free</u> </strong></em></p><p><em><strong></strong></em></p><p><em><strong>*<em><strong>An expert panel of the Endocrine Society (9) reviewed the various methods for determining free testosterone (4,5,9,21-32) and </strong></em></strong><em><strong>concluded that each method has some inherent limitations but that the <u>equilibrium dialysis method is the reference standard against which all other methods should be compared</u> (33). <u>However, substantial heterogeneity in the procedures used by various laboratories for performing the equilibrium dialysis assay has contributed to variability in the reported free testosterone values</u></strong></em></em></p><p></p><p><strong>*</strong><em><strong>Furthermore, the determination of free testosterone concentration by equilibrium dialysis is affected greatly by the assay conditions, including the <u>buffer composition, the incubation time, and the temperature</u></strong></em></p><p><em><strong></strong></em></p><p><em><strong>*</strong><em><strong>The accuracy and precision of the total testosterone assay also affect the precision and accuracy of the measured free testosterone concentration. <u>Most commercial laboratories do not report the buffer composition and other dialysis conditions which renders it difficult to evaluate their methods; the procedures for equilibrium dialysis have varied even in published reports from academic research laboratories</u> (2,24,32-37). <u>Because of the wide variation in the equilibrium dialysis procedures, the reference ranges are not generalizable across laboratories</u></strong></em></em></p></blockquote><p></p>
[QUOTE="madman, post: 236368, member: 13851"] [I][B]*Most of the circulating testosterone is bound to sex hormone-binding globulin (SHBG) and human serum albumin (HSA), and a smaller fraction is bound to cortisol-binding globulin and orosomucoid; [U]only less than 4.0% of circulating testosterone is unbound or free[/U] *[I][B]An expert panel of the Endocrine Society (9) reviewed the various methods for determining free testosterone (4,5,9,21-32) and [/B][/I][/B][I][B]concluded that each method has some inherent limitations but that the [U]equilibrium dialysis method is the reference standard against which all other methods should be compared[/U] (33). [U]However, substantial heterogeneity in the procedures used by various laboratories for performing the equilibrium dialysis assay has contributed to variability in the reported free testosterone values[/U][/B][/I][/I] [B]*[/B][I][B]Furthermore, the determination of free testosterone concentration by equilibrium dialysis is affected greatly by the assay conditions, including the [U]buffer composition, the incubation time, and the temperature[/U] *[/B][I][B]The accuracy and precision of the total testosterone assay also affect the precision and accuracy of the measured free testosterone concentration. [U]Most commercial laboratories do not report the buffer composition and other dialysis conditions which renders it difficult to evaluate their methods; the procedures for equilibrium dialysis have varied even in published reports from academic research laboratories[/U] (2,24,32-37). [U]Because of the wide variation in the equilibrium dialysis procedures, the reference ranges are not generalizable across laboratories[/U][/B][/I][/I] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Reference Intervals for Free Testosterone in Adult Men Measured Using a Standardized Equilibrium Dialysis Procedure
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