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PectaSol-C modified citrus pectin induces apoptosis and inhibition of proliferation in androgen-dependent and- independent prostate cancer
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<blockquote data-quote="ajax31" data-source="post: 255352" data-attributes="member: 39208"><p>[URL unfurl="true"]https://pubmed.ncbi.nlm.nih.gov/20462856/[/URL]</p><p></p><p>[PectaSol-C also effective for BPH]</p><h3>PectaSol-C modified citrus pectin induces apoptosis and inhibition of proliferation in human and mouse androgen-dependent and- independent prostate cancer cells</h3><p><a href="https://pubmed.ncbi.nlm.nih.gov/?term=Yan+J&cauthor_id=20462856" target="_blank">Jun Yan</a> <a href="https://pubmed.ncbi.nlm.nih.gov/20462856/#full-view-affiliation-1" target="_blank">1</a>, <a href="https://pubmed.ncbi.nlm.nih.gov/?term=Katz+A&cauthor_id=20462856" target="_blank">Aaron Katz</a></p><p>Affiliations expand</p><ul> <li data-xf-list-type="ul">PMID: <strong>20462856</strong></li> <li data-xf-list-type="ul">DOI: <a href="https://doi.org/10.1177/1534735410369672" target="_blank">10.1177/1534735410369672</a></li> </ul><p>Free article</p><h3>Abstract</h3><p><strong>Aim: </strong>To demonstrate the efficacy of PectaSol-C modified citrus pectin (MCP) on prostate cancer in vitro.</p><p><strong>Method: </strong>Cytotoxicity analysis of PectaSol-C was performed by MTT assay, as were parallel studies with the former brand version of MCP called PectaSol. Apoptosis and inhibition of cell growth were investigated by Western blotting.</p><p><strong>Results: </strong>Androgen-dependent and -independent human prostate cancer cell lines (LNCaP and PC3, respectively), androgen-dependent and -independent murine prostate cancer cell lines (CASP2.1 and CASP1.1, respectively), as well as noncancerous human benign prostate hyperplasia BPH-1 cell line, were used in the study. MTT assay revealed that 1.0% PectaSol exerted cytotoxicity on LNCaP, PC3, CASP2.1, CASP1.1, and BPH-1 cells for 4-day treatment by 48.0% +/- 2.1%, 54.4% +/- 0.3%, 15.4% +/- 0.8%, 46.1% +/- 1.7%, and 27.4% +/- 1.6%, respectively; whereas 1.0% PectaSol-C showed cytotoxity by 52.2% +/- 1.8%, 48.2% +/- 2.9%, 23.0% +/- 2.6%, 49.0% +/- 1.3%, and 26.8% +/- 2.6%, respectively. Western blotting further confirmed that both MCPs inhibit MAP kinase activation, increase the expression level of its downstream target Bim, a pro-apoptotic protein, and induce the cleavage of Caspase-3 in PC3 and CASP1.1 prostate cancer cells.</p><p><strong>Conclusion: </strong>PectaSol MCP and PectaSol-C MCP can inhibit cell proliferation and apoptosis in prostate cancer cell lines. Our data suggested that 1.0% PectaSol-C can be used for further chemopreventive and chemotherapeutic analysis in vivo.</p></blockquote><p></p>
[QUOTE="ajax31, post: 255352, member: 39208"] [URL unfurl="true"]https://pubmed.ncbi.nlm.nih.gov/20462856/[/URL] [PectaSol-C also effective for BPH] [HEADING=2]PectaSol-C modified citrus pectin induces apoptosis and inhibition of proliferation in human and mouse androgen-dependent and- independent prostate cancer cells[/HEADING] [URL='https://pubmed.ncbi.nlm.nih.gov/?term=Yan+J&cauthor_id=20462856']Jun Yan[/URL] [URL='https://pubmed.ncbi.nlm.nih.gov/20462856/#full-view-affiliation-1']1[/URL], [URL='https://pubmed.ncbi.nlm.nih.gov/?term=Katz+A&cauthor_id=20462856']Aaron Katz[/URL] Affiliations expand [LIST] [*]PMID: [B]20462856[/B] [*]DOI: [URL='https://doi.org/10.1177/1534735410369672']10.1177/1534735410369672[/URL] [/LIST] Free article [HEADING=2]Abstract[/HEADING] [B]Aim: [/B]To demonstrate the efficacy of PectaSol-C modified citrus pectin (MCP) on prostate cancer in vitro. [B]Method: [/B]Cytotoxicity analysis of PectaSol-C was performed by MTT assay, as were parallel studies with the former brand version of MCP called PectaSol. Apoptosis and inhibition of cell growth were investigated by Western blotting. [B]Results: [/B]Androgen-dependent and -independent human prostate cancer cell lines (LNCaP and PC3, respectively), androgen-dependent and -independent murine prostate cancer cell lines (CASP2.1 and CASP1.1, respectively), as well as noncancerous human benign prostate hyperplasia BPH-1 cell line, were used in the study. MTT assay revealed that 1.0% PectaSol exerted cytotoxicity on LNCaP, PC3, CASP2.1, CASP1.1, and BPH-1 cells for 4-day treatment by 48.0% +/- 2.1%, 54.4% +/- 0.3%, 15.4% +/- 0.8%, 46.1% +/- 1.7%, and 27.4% +/- 1.6%, respectively; whereas 1.0% PectaSol-C showed cytotoxity by 52.2% +/- 1.8%, 48.2% +/- 2.9%, 23.0% +/- 2.6%, 49.0% +/- 1.3%, and 26.8% +/- 2.6%, respectively. Western blotting further confirmed that both MCPs inhibit MAP kinase activation, increase the expression level of its downstream target Bim, a pro-apoptotic protein, and induce the cleavage of Caspase-3 in PC3 and CASP1.1 prostate cancer cells. [B]Conclusion: [/B]PectaSol MCP and PectaSol-C MCP can inhibit cell proliferation and apoptosis in prostate cancer cell lines. Our data suggested that 1.0% PectaSol-C can be used for further chemopreventive and chemotherapeutic analysis in vivo. [/QUOTE]
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PectaSol-C modified citrus pectin induces apoptosis and inhibition of proliferation in androgen-dependent and- independent prostate cancer
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