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Interesting—that seems like a high peak for a 500 ng/dL trough on E3.5D injections. Let's just say it is 900 ng/dL and your average level is 700 ng/dL. The effects of EOD dosing are harder to predict. For me it seems to result in little variation. But you may be a faster absorber and still see pronounced peaks and troughs in serum testosterone. For the case with little variation you'd simply be scaling the average level of 700 ng/dL down to 500 ng/dL. The EOD dose is 100 / 3.5 *5 / 7 mg = 20 mg, just as you predicted. If you have some variability with EOD dosing then a little higher dose would be necessary to maintain the 500 ng/dL trough. On the other hand, if your current peaks are much over 900 ng/dL then a lower dose could be appropriate. In any case, these are pretty crude estimates, so a need for later tuning is to be expected.