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I think to get a semi-decent estimate of your peak value you need to know the expected average along with the pre-injection trough value. Basically you model the situation with serum testosterone as a linear rise to the peak followed by a linear fall to the trough. Then you assume the peak is as much over the average as the trough is below.

You have a single non-trough value, and the addition of hCG is confounding if it's stimulating production of a non-negligible amount of endogenous testosterone. If the hCG contribution is small then you might estimate a trough testosterone level by assuming the 0.8-day half-life over eight hours. This drops the 495 measurement down to about 370.