ExcelMale
Menu
Home
What's new
Latest activity
Forums
New posts
Search forums
What's new
New posts
Latest activity
Videos
Lab Tests
Doctor Finder
Buy Books
About Us
Men’s Health Coaching
Log in
Register
What's new
Search
Search
Search titles only
By:
New posts
Search forums
Menu
Log in
Register
Navigation
Install the app
Install
More options
Contact us
Close Menu
Forums
Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Normative Basal Values of Hormones and Proteins of Gonadal and Adrenal Functions from Birth to Adulthood
JavaScript is disabled. For a better experience, please enable JavaScript in your browser before proceeding.
You are using an out of date browser. It may not display this or other websites correctly.
You should upgrade or use an
alternative browser
.
Reply to thread
Message
<blockquote data-quote="madman" data-source="post: 147751" data-attributes="member: 13851"><p><strong>Abstract </strong></p><p>In clinical practice, it is fundamental to compare the results of hormonal examinations obtained in the laboratory with reliable reference values. This is particularly difficult when faced with rare conditions, such as disorders of sex development, where not routinely assayed peptide hormones as well as intermediate steroid metabolites are often needed and local reliable reference values are not available. <strong>There are considerable differences among <span style="color: rgb(184, 49, 47)">techniques and assays</span> used in clinical and research laboratories.</strong> <strong>In fact, laboratory hormonology is undergoing a critical transition between <span style="color: rgb(184, 49, 47)">techniques for quantitative determination: established immunoassays and mass spectrometry.</span> <span style="color: rgb(0, 0, 0)">Harmonizing results </span>from different laboratories is <span style="color: rgb(184, 49, 47)">a major challenge </span>along the path leading to the establishment of <span style="color: rgb(184, 49, 47)">consensus reference intervals for steroid hormones.</span></strong> <strong>Most of the efforts are being <span style="color: rgb(184, 49, 47)">concentrated on testosterone</span>, with very encouraging results being provided by the <span style="color: rgb(184, 49, 47)">harmonization of liquid chromatography-tandem mass spectrometry results.</span> However, this goal is still <span style="color: rgb(184, 49, 47)">far from being achieved</span> for the other <span style="color: rgb(184, 49, 47)">steroid and small-molecule hormones</span>, and a much more challenging perspective is foreseeable for <span style="color: rgb(184, 49, 47)">protein hormones.</span></strong> <strong>In addition to <span style="color: rgb(184, 49, 47)">technical issues</span>, the importance of the<span style="color: rgb(184, 49, 47)"> definition and of the characterization </span>of the<span style="color: rgb(184, 49, 47)"> reference population</span> as well as <span style="color: rgb(184, 49, 47)">sampling and processing methodology</span> should not be underestimated, as these<span style="color: rgb(184, 49, 47)"> aspects </span>may impact on <span style="color: rgb(184, 49, 47)">hormonal axis and compound stability.</span></strong> <span style="color: rgb(0, 0, 0)"><strong>The aim of the present review is to </strong></span><span style="color: rgb(184, 49, 47)"><strong>provide a comprehensive overview </strong></span><span style="color: rgb(0, 0, 0)"><strong>of the </strong></span><span style="color: rgb(184, 49, 47)"><strong>circulating reference values</strong></span><span style="color: rgb(0, 0, 0)"><strong> in </strong></span><span style="color: rgb(184, 49, 47)"><strong>basal condition </strong></span><span style="color: rgb(0, 0, 0)"><strong>of the </strong></span><span style="color: rgb(184, 49, 47)"><strong>hormones and proteins </strong></span><span style="color: rgb(0, 0, 0)"><strong>involved in </strong></span><span style="color: rgb(184, 49, 47)"><strong>sex development </strong></span><span style="color: rgb(0, 0, 0)"><strong>reported to date in the </strong></span><span style="color: rgb(184, 49, 47)"><strong>peer reviewed literature. </strong></span><span style="color: rgb(0, 0, 0)"><strong>We present a series of tables where we have collected the </strong></span><span style="color: rgb(184, 49, 47)"><strong>reference intervals</strong></span><span style="color: rgb(0, 0, 0)"><strong> for each </strong></span><span style="color: rgb(184, 49, 47)"><strong>specific hormone and protein.</strong></span></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p><strong><span style="color: rgb(184, 49, 47)">Laboratory hormonology</span> is undergoing a <span style="color: rgb(184, 49, 47)">critical period of transition</span> between <span style="color: rgb(184, 49, 47)">milestone techniques</span> for <span style="color: rgb(184, 49, 47)">quantitative determination:</span> <span style="color: rgb(44, 130, 201)">established immunoassays, mainly in their direct and automated versions,</span> and <span style="color: rgb(184, 49, 47)">mass spectrometry in its latest hyphenation with liquid chromatography (LC-MS/MS) [Taylor et al., 2015].</span></strong> <strong>There are<span style="color: rgb(184, 49, 47)"> clear differences </span>between the <span style="color: rgb(184, 49, 47)">2 techniques</span> in terms of <span style="color: rgb(184, 49, 47)">analytical principles, benefits, drawbacks, and potential,</span> which have been extensively described elsewhere in the present issue. </strong></p><p></p><p></p><p><strong><span style="color: rgb(184, 49, 47)">LC-MS/MS</span> is seen as the<span style="color: rgb(184, 49, 47)"> first real opportunity </span>to achieve <span style="color: rgb(184, 49, 47)">a global harmonization of results</span> generated across laboratories worldwide. </strong>Considerable advancements have been achieved, as promoted by health agencies as well as scientific communities [Rosner et al., 2007, 2013; Vesper et al., 2009; Auchus, 2014; Wierman et al., 2014; Büttler et al., 2015, 2016]. <strong>The recent initiative of the <span style="color: rgb(184, 49, 47)">Endocrine Society on the harmonization </span>of <span style="color: rgb(184, 49, 47)">results from large epidemiologic studies</span> on <span style="color: rgb(184, 49, 47)">male hypogonadism </span>represents <span style="color: rgb(184, 49, 47)">a milestone</span>, <span style="color: rgb(44, 130, 201)">as for the first time it established consensus reference intervals for testosterone [Travison et al., 2017].</span></strong> <strong>Nonetheless, this goal is still far from being achieved for <span style="color: rgb(184, 49, 47)">the other steroid and small-molecule hormones</span>, and a much more <span style="color: rgb(184, 49, 47)">challenging perspective</span> is foreseeable for <span style="color: rgb(184, 49, 47)">protein hormones [Sabbagh et al., 2016]. </span><span style="color: rgb(44, 130, 201)">The techniques and assays used in clinical and research laboratories differ considerably. </span>The choice of assay is too often <span style="color: rgb(184, 49, 47)">dominated by convenience and financial reasons </span>but rarely by an evaluation of the <span style="color: rgb(184, 49, 47)">quality and reliability of the results.</span> This situation is further exacerbated by the <span style="color: rgb(184, 49, 47)">high workload faced by the laboratories.</span></strong> <strong>Thus, it is of <span style="color: rgb(184, 49, 47)">key importance </span>that each laboratory uses <span style="color: rgb(184, 49, 47)">assay-specific reference values.</span></strong></p><p></p><p></p><p>Nonetheless, the accuracy of reference intervals does not only rely on the assay used, since important aspects concerning the “reference” definition for the cohort and the sampling conditions need to be taken into account when generating normative limits, as well as when interpreting patients’ results according to these normative levels. Reference values provided by ready-to-use kit vendors do not provide exhaustive information on such issues. On the other hand, it is very difficult for laboratories to have the resources to build up their own values [Fanelli et al., 2013a]</p><p></p><p></p><p><strong>The aim of the present review is to provide <span style="color: rgb(184, 49, 47)">a comprehensive overview</span> of the <span style="color: rgb(184, 49, 47)">circulating reference values </span>in basal conditions of <span style="color: rgb(184, 49, 47)">hormones involved in sex development</span> and of <span style="color: rgb(184, 49, 47)">sex hormone binding globulin </span>reported to date in the <span style="color: rgb(184, 49, 47)">peer-reviewed literature.</span></strong></p><p></p><p></p><p></p><p></p><p></p><p><strong><span style="color: rgb(184, 49, 47)">Table 2. </span> Total testosterone (TT) reference intervals according to age and testicular volume in the male population</strong></p><p></p><p>[ATTACH=full]7411[/ATTACH]</p><p></p><p></p><p></p><p></p><p></p><p><strong><span style="color: rgb(184, 49, 47)">Table 8. </span> Sex hormone binding globulin (SHBG) reference intervals according to age in the male population</strong></p><p></p><p>[ATTACH=full]7412[/ATTACH]</p><p></p><p></p><p></p><p></p><p></p><p><strong><span style="color: rgb(184, 49, 47)">Table 10. </span> Dihydrotestosterone (DHT) reference intervals according to age and testicular volume in the male population</strong></p><p></p><p>[ATTACH=full]7413[/ATTACH]</p><p></p><p></p><p></p><p></p><p></p><p><strong><span style="color: rgb(184, 49, 47)">Table 14. </span> Dehydroepiandrosterone (DHEA) reference intervals according to age and Tanner stage in the male population</strong></p><p></p><p>[ATTACH=full]7414[/ATTACH]</p><p></p><p></p><p></p><p></p><p></p><p><strong><span style="color: rgb(209, 72, 65)">Table 3</span><span style="color: rgb(184, 49, 47)">6.</span> Estradiol (E2) and estrone (E1) reference intervals according to age and Tanner stage in the male population</strong></p><p></p><p>[ATTACH=full]7415[/ATTACH]</p><p></p><p>[ATTACH=full]7416[/ATTACH]</p></blockquote><p></p>
[QUOTE="madman, post: 147751, member: 13851"] [B]Abstract [/B] In clinical practice, it is fundamental to compare the results of hormonal examinations obtained in the laboratory with reliable reference values. This is particularly difficult when faced with rare conditions, such as disorders of sex development, where not routinely assayed peptide hormones as well as intermediate steroid metabolites are often needed and local reliable reference values are not available. [B]There are considerable differences among [COLOR=rgb(184, 49, 47)]techniques and assays[/COLOR] used in clinical and research laboratories.[/B] [B]In fact, laboratory hormonology is undergoing a critical transition between [COLOR=rgb(184, 49, 47)]techniques for quantitative determination: established immunoassays and mass spectrometry.[/COLOR] [COLOR=rgb(0, 0, 0)]Harmonizing results [/COLOR]from different laboratories is [COLOR=rgb(184, 49, 47)]a major challenge [/COLOR]along the path leading to the establishment of [COLOR=rgb(184, 49, 47)]consensus reference intervals for steroid hormones.[/COLOR][/B] [B]Most of the efforts are being [COLOR=rgb(184, 49, 47)]concentrated on testosterone[/COLOR], with very encouraging results being provided by the [COLOR=rgb(184, 49, 47)]harmonization of liquid chromatography-tandem mass spectrometry results.[/COLOR] However, this goal is still [COLOR=rgb(184, 49, 47)]far from being achieved[/COLOR] for the other [COLOR=rgb(184, 49, 47)]steroid and small-molecule hormones[/COLOR], and a much more challenging perspective is foreseeable for [COLOR=rgb(184, 49, 47)]protein hormones.[/COLOR][/B] [B]In addition to [COLOR=rgb(184, 49, 47)]technical issues[/COLOR], the importance of the[COLOR=rgb(184, 49, 47)] definition and of the characterization [/COLOR]of the[COLOR=rgb(184, 49, 47)] reference population[/COLOR] as well as [COLOR=rgb(184, 49, 47)]sampling and processing methodology[/COLOR] should not be underestimated, as these[COLOR=rgb(184, 49, 47)] aspects [/COLOR]may impact on [COLOR=rgb(184, 49, 47)]hormonal axis and compound stability.[/COLOR][/B] [COLOR=rgb(0, 0, 0)][B]The aim of the present review is to [/B][/COLOR][COLOR=rgb(184, 49, 47)][B]provide a comprehensive overview [/B][/COLOR][COLOR=rgb(0, 0, 0)][B]of the [/B][/COLOR][COLOR=rgb(184, 49, 47)][B]circulating reference values[/B][/COLOR][COLOR=rgb(0, 0, 0)][B] in [/B][/COLOR][COLOR=rgb(184, 49, 47)][B]basal condition [/B][/COLOR][COLOR=rgb(0, 0, 0)][B]of the [/B][/COLOR][COLOR=rgb(184, 49, 47)][B]hormones and proteins [/B][/COLOR][COLOR=rgb(0, 0, 0)][B]involved in [/B][/COLOR][COLOR=rgb(184, 49, 47)][B]sex development [/B][/COLOR][COLOR=rgb(0, 0, 0)][B]reported to date in the [/B][/COLOR][COLOR=rgb(184, 49, 47)][B]peer reviewed literature. [/B][/COLOR][COLOR=rgb(0, 0, 0)][B]We present a series of tables where we have collected the [/B][/COLOR][COLOR=rgb(184, 49, 47)][B]reference intervals[/B][/COLOR][COLOR=rgb(0, 0, 0)][B] for each [/B][/COLOR][COLOR=rgb(184, 49, 47)][B]specific hormone and protein.[/B][/COLOR] [B][COLOR=rgb(184, 49, 47)]Laboratory hormonology[/COLOR] is undergoing a [COLOR=rgb(184, 49, 47)]critical period of transition[/COLOR] between [COLOR=rgb(184, 49, 47)]milestone techniques[/COLOR] for [COLOR=rgb(184, 49, 47)]quantitative determination:[/COLOR] [COLOR=rgb(44, 130, 201)]established immunoassays, mainly in their direct and automated versions,[/COLOR] and [COLOR=rgb(184, 49, 47)]mass spectrometry in its latest hyphenation with liquid chromatography (LC-MS/MS) [Taylor et al., 2015].[/COLOR][/B] [B]There are[COLOR=rgb(184, 49, 47)] clear differences [/COLOR]between the [COLOR=rgb(184, 49, 47)]2 techniques[/COLOR] in terms of [COLOR=rgb(184, 49, 47)]analytical principles, benefits, drawbacks, and potential,[/COLOR] which have been extensively described elsewhere in the present issue. [/B] [B][COLOR=rgb(184, 49, 47)]LC-MS/MS[/COLOR] is seen as the[COLOR=rgb(184, 49, 47)] first real opportunity [/COLOR]to achieve [COLOR=rgb(184, 49, 47)]a global harmonization of results[/COLOR] generated across laboratories worldwide. [/B]Considerable advancements have been achieved, as promoted by health agencies as well as scientific communities [Rosner et al., 2007, 2013; Vesper et al., 2009; Auchus, 2014; Wierman et al., 2014; Büttler et al., 2015, 2016]. [B]The recent initiative of the [COLOR=rgb(184, 49, 47)]Endocrine Society on the harmonization [/COLOR]of [COLOR=rgb(184, 49, 47)]results from large epidemiologic studies[/COLOR] on [COLOR=rgb(184, 49, 47)]male hypogonadism [/COLOR]represents [COLOR=rgb(184, 49, 47)]a milestone[/COLOR], [COLOR=rgb(44, 130, 201)]as for the first time it established consensus reference intervals for testosterone [Travison et al., 2017].[/COLOR][/B] [B]Nonetheless, this goal is still far from being achieved for [COLOR=rgb(184, 49, 47)]the other steroid and small-molecule hormones[/COLOR], and a much more [COLOR=rgb(184, 49, 47)]challenging perspective[/COLOR] is foreseeable for [COLOR=rgb(184, 49, 47)]protein hormones [Sabbagh et al., 2016]. [/COLOR][COLOR=rgb(44, 130, 201)]The techniques and assays used in clinical and research laboratories differ considerably. [/COLOR]The choice of assay is too often [COLOR=rgb(184, 49, 47)]dominated by convenience and financial reasons [/COLOR]but rarely by an evaluation of the [COLOR=rgb(184, 49, 47)]quality and reliability of the results.[/COLOR] This situation is further exacerbated by the [COLOR=rgb(184, 49, 47)]high workload faced by the laboratories.[/COLOR][/B] [B]Thus, it is of [COLOR=rgb(184, 49, 47)]key importance [/COLOR]that each laboratory uses [COLOR=rgb(184, 49, 47)]assay-specific reference values.[/COLOR][/B] Nonetheless, the accuracy of reference intervals does not only rely on the assay used, since important aspects concerning the “reference” definition for the cohort and the sampling conditions need to be taken into account when generating normative limits, as well as when interpreting patients’ results according to these normative levels. Reference values provided by ready-to-use kit vendors do not provide exhaustive information on such issues. On the other hand, it is very difficult for laboratories to have the resources to build up their own values [Fanelli et al., 2013a] [B]The aim of the present review is to provide [COLOR=rgb(184, 49, 47)]a comprehensive overview[/COLOR] of the [COLOR=rgb(184, 49, 47)]circulating reference values [/COLOR]in basal conditions of [COLOR=rgb(184, 49, 47)]hormones involved in sex development[/COLOR] and of [COLOR=rgb(184, 49, 47)]sex hormone binding globulin [/COLOR]reported to date in the [COLOR=rgb(184, 49, 47)]peer-reviewed literature.[/COLOR][/B] [B][COLOR=rgb(184, 49, 47)]Table 2. [/COLOR] Total testosterone (TT) reference intervals according to age and testicular volume in the male population[/B] [ATTACH=full]7411[/ATTACH] [B][COLOR=rgb(184, 49, 47)]Table 8. [/COLOR] Sex hormone binding globulin (SHBG) reference intervals according to age in the male population[/B] [ATTACH=full]7412[/ATTACH] [B][COLOR=rgb(184, 49, 47)]Table 10. [/COLOR] Dihydrotestosterone (DHT) reference intervals according to age and testicular volume in the male population[/B] [ATTACH=full]7413[/ATTACH] [B][COLOR=rgb(184, 49, 47)]Table 14. [/COLOR] Dehydroepiandrosterone (DHEA) reference intervals according to age and Tanner stage in the male population[/B] [ATTACH=full]7414[/ATTACH] [B][COLOR=rgb(209, 72, 65)]Table 3[/COLOR][COLOR=rgb(184, 49, 47)]6.[/COLOR] Estradiol (E2) and estrone (E1) reference intervals according to age and Tanner stage in the male population[/B] [ATTACH=full]7415[/ATTACH] [ATTACH=full]7416[/ATTACH] [/QUOTE]
Insert quotes…
Verification
Post reply
Share this page
Facebook
Twitter
Reddit
Pinterest
Tumblr
WhatsApp
Email
Share
Link
Sponsors
Forums
Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Normative Basal Values of Hormones and Proteins of Gonadal and Adrenal Functions from Birth to Adulthood
This site uses cookies to help personalise content, tailor your experience and to keep you logged in if you register.
By continuing to use this site, you are consenting to our use of cookies.
Accept
Learn more…
Top