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General Health & Fitness
Nutrition and Supplements
Mice Fed Fatty Diets For 30 Weeks Show Signs of Depression, Anxiety, And Alzheimer's
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<blockquote data-quote="tareload" data-source="post: 227994"><p>I don't have access to article mentioned in title of thread but here's an example of "high fat" diet sometimes used in the rodent literature:</p><p></p><p>[URL unfurl="true"]https://academic.oup.com/cdn/article/4/Supplement_2/87/5844998[/URL]</p><p></p><p></p><p>Methods</p><p>Seven-week-old male <em>Apoe</em>−/− mice, a model for human atherosclerosis, were fed <em>ad libitum</em> (%kcal) <strong>KD (81-fat, 1-carbohydrate, 18-protein; <em>n </em>= 4) or HF (40-fat, 42-carbohydrate, 18-protein; <em>n </em>= 5). </strong>After 4, 8 and 12 weeks, plasma was collected and used to (1) quantify beta-hydroxybutyrate levels (OH-But) by a colorimetric assay; or (2) assess systemic inflammation, a key feature associated with atherosclerosis, using a panel of inflammatory cytokines; or (3) explore diet-driven changes in levels of atherosclerosis-relevant metabolites using a targeted metabolomic approach by triple quadrupole mass spectrometry. At the endpoint, mice were euthanized and their perfusion-fixed aortas were subjected to 3-D analysis by magnetic resonance imaging to quantify the extent of atherosclerosis. Data were reconstructed using Matlab and segmented to obtain atherosclerotic plaque volumes using Avizo 9.0.</p><p></p><p></p><p>High fat diet lingo in the rodent literature is code for standard American diet (2:2:1 ratio of % of kcal for F/C/P). Ketogenic diet (KD) above is actually the true high fat diet <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite1" alt=":)" title="Smile :)" loading="lazy" data-shortname=":)" />!</p></blockquote><p></p>
[QUOTE="tareload, post: 227994"] I don't have access to article mentioned in title of thread but here's an example of "high fat" diet sometimes used in the rodent literature: [URL unfurl="true"]https://academic.oup.com/cdn/article/4/Supplement_2/87/5844998[/URL] Methods Seven-week-old male [I]Apoe[/I]−/− mice, a model for human atherosclerosis, were fed [I]ad libitum[/I] (%kcal) [B]KD (81-fat, 1-carbohydrate, 18-protein; [I]n [/I]= 4) or HF (40-fat, 42-carbohydrate, 18-protein; [I]n [/I]= 5). [/B]After 4, 8 and 12 weeks, plasma was collected and used to (1) quantify beta-hydroxybutyrate levels (OH-But) by a colorimetric assay; or (2) assess systemic inflammation, a key feature associated with atherosclerosis, using a panel of inflammatory cytokines; or (3) explore diet-driven changes in levels of atherosclerosis-relevant metabolites using a targeted metabolomic approach by triple quadrupole mass spectrometry. At the endpoint, mice were euthanized and their perfusion-fixed aortas were subjected to 3-D analysis by magnetic resonance imaging to quantify the extent of atherosclerosis. Data were reconstructed using Matlab and segmented to obtain atherosclerotic plaque volumes using Avizo 9.0. High fat diet lingo in the rodent literature is code for standard American diet (2:2:1 ratio of % of kcal for F/C/P). Ketogenic diet (KD) above is actually the true high fat diet :-)! [/QUOTE]
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General Health & Fitness
Nutrition and Supplements
Mice Fed Fatty Diets For 30 Weeks Show Signs of Depression, Anxiety, And Alzheimer's
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