Figure 3: Graphical representation, in evaluated concentration order, of differences between testosterone liquid chromatography coupled to the tandem mass spectrometry (LC-MS/MS) methods and the various immunoassays for five serum samples labeled A–E. Upper panel: Six immunoassay’s analytical bias (A) and relative bias (B) estimated using the mean value of three LC-MS/MS. Each symbol depicts the testosterone mean value of each method’s results with standard deviation error bars. ■ = Abbot Alinity I (n=1), ● = Beckman UniCel DxI 800 (n=2), ; = Cobas Roche e601/e801 analyzer series and e411 analyzer (n=11), : = Ortho Vitros 3,600 (n=1),A= Siemens Avida Centaur (n=1), = = Cisbio CT RIA (n=1). One result from Ortho Vitros and one from Siemens Avida Centaur, were designated as outliers (reported values <0.49 nmol/L and <0.24 nmol/L at 0.06 nmol/L) and therefore omitted in the graphic presentation, panel B. Lower panel: detailed plot of analytical bias (C) and relative bias (D) for the eleven Cobas Roche e601/e801 analyzer series and e411 analyzer, estimated using the mean value of three LC-MS/MS. Each connected line represents one laboratory’s testosterone results. One laboratory result was designated as an outlier (217 % relative bias at 0.06 nmol/L) and therefore omitted in the graphic presentation.
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