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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Methodological considerations in determining sex steroids in children
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<blockquote data-quote="madman" data-source="post: 262079" data-attributes="member: 13851"><p><strong>Figure 3: Graphical representation, in evaluated concentration order, of differences between testosterone liquid chromatography coupled to the tandem mass spectrometry (LC-MS/MS) methods and the various immunoassays for five serum samples labeled A–E.</strong> Upper panel: Six immunoassay’s analytical bias (A) and relative bias (B) estimated using the mean value of three LC-MS/MS. Each symbol depicts the testosterone mean value of each method’s results with standard deviation error bars. ■ = Abbot Alinity I (n=1), ● = Beckman UniCel DxI 800 (n=2), ; = Cobas Roche e601/e801 analyzer series and e411 analyzer (n=11), : = Ortho Vitros 3,600 (n=1),A= Siemens Avida Centaur (n=1), = = Cisbio CT RIA (n=1). One result from Ortho Vitros and one from Siemens Avida Centaur, were designated as outliers (reported values <0.49 nmol/L and <0.24 nmol/L at 0.06 nmol/L) and therefore omitted in the graphic presentation, panel B. Lower panel: detailed plot of analytical bias (C) and relative bias (D) for the eleven Cobas Roche e601/e801 analyzer series and e411 analyzer, estimated using the mean value of three LC-MS/MS. Each connected line represents one laboratory’s testosterone results. One laboratory result was designated as an outlier (217 % relative bias at 0.06 nmol/L) and therefore omitted in the graphic presentation.</p><p>[ATTACH=full]35645[/ATTACH]</p></blockquote><p></p>
[QUOTE="madman, post: 262079, member: 13851"] [B]Figure 3: Graphical representation, in evaluated concentration order, of differences between testosterone liquid chromatography coupled to the tandem mass spectrometry (LC-MS/MS) methods and the various immunoassays for five serum samples labeled A–E.[/B] Upper panel: Six immunoassay’s analytical bias (A) and relative bias (B) estimated using the mean value of three LC-MS/MS. Each symbol depicts the testosterone mean value of each method’s results with standard deviation error bars. ■ = Abbot Alinity I (n=1), ● = Beckman UniCel DxI 800 (n=2), ; = Cobas Roche e601/e801 analyzer series and e411 analyzer (n=11), : = Ortho Vitros 3,600 (n=1),A= Siemens Avida Centaur (n=1), = = Cisbio CT RIA (n=1). One result from Ortho Vitros and one from Siemens Avida Centaur, were designated as outliers (reported values <0.49 nmol/L and <0.24 nmol/L at 0.06 nmol/L) and therefore omitted in the graphic presentation, panel B. Lower panel: detailed plot of analytical bias (C) and relative bias (D) for the eleven Cobas Roche e601/e801 analyzer series and e411 analyzer, estimated using the mean value of three LC-MS/MS. Each connected line represents one laboratory’s testosterone results. One laboratory result was designated as an outlier (217 % relative bias at 0.06 nmol/L) and therefore omitted in the graphic presentation. [ATTACH type="full"]35645[/ATTACH] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Methodological considerations in determining sex steroids in children
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