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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Methodological considerations in determining sex steroids in children
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<blockquote data-quote="madman" data-source="post: 262078" data-attributes="member: 13851"><p><strong>Figure 2: Graphical representation of differences in estradiol quantitation, in order of evaluated concentration, in five child samples labelled A–E, between various immunoassays and liquid chromatography tandem mass spectrometry (LC-MS/MS) methods. </strong>Upper panel: Six immunoassays’ analytical biases (A) and relative biases (B) estimated using the mean value of three LC-MS/MS. Each symbol represents the estradiol mean value of each method’s results with standard deviations as error bars. ■ = Abbot Alinity I (n=1), ● = Beckman UniCel DxI 800 (n=2),;= Cobas Roche e601/e801 analyzer series and e411 analyzer (n=10),:= Ortho Vitros 3,600 (n=1),A= Siemens Avida Centaur (n=2),== Cisbio CT RIA + in-house extraction (n=1). Two results from Abbot Alinity, were designated as outliers (1,591 % relative bias at 4.9 and 1,303 % at 8.5 pmol/L) and therefore omitted in the graphic presentation. Lower panel: Detailed plot of analytical bias (C) and relative bias (D) for ten Cobas Roche e601/e801 analyzer series and e411 analyzer, estimated using the mean value of three LC-MS/MS. Each connected line represents one laboratory’s estradiol results</p><p>[ATTACH=full]35644[/ATTACH]</p></blockquote><p></p>
[QUOTE="madman, post: 262078, member: 13851"] [B]Figure 2: Graphical representation of differences in estradiol quantitation, in order of evaluated concentration, in five child samples labelled A–E, between various immunoassays and liquid chromatography tandem mass spectrometry (LC-MS/MS) methods. [/B]Upper panel: Six immunoassays’ analytical biases (A) and relative biases (B) estimated using the mean value of three LC-MS/MS. Each symbol represents the estradiol mean value of each method’s results with standard deviations as error bars. ■ = Abbot Alinity I (n=1), ● = Beckman UniCel DxI 800 (n=2),;= Cobas Roche e601/e801 analyzer series and e411 analyzer (n=10),:= Ortho Vitros 3,600 (n=1),A= Siemens Avida Centaur (n=2),== Cisbio CT RIA + in-house extraction (n=1). Two results from Abbot Alinity, were designated as outliers (1,591 % relative bias at 4.9 and 1,303 % at 8.5 pmol/L) and therefore omitted in the graphic presentation. Lower panel: Detailed plot of analytical bias (C) and relative bias (D) for ten Cobas Roche e601/e801 analyzer series and e411 analyzer, estimated using the mean value of three LC-MS/MS. Each connected line represents one laboratory’s estradiol results [ATTACH type="full"]35644[/ATTACH] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Methodological considerations in determining sex steroids in children
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