ExcelMale
Menu
Home
What's new
Latest activity
Forums
New posts
Search forums
What's new
New posts
Latest activity
Videos
Lab Tests
Doctor Finder
Buy Books
About Us
Men’s Health Coaching
Log in
Register
What's new
Search
Search
Search titles only
By:
New posts
Search forums
Menu
Log in
Register
Navigation
Install the app
Install
More options
Contact us
Close Menu
Forums
Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Basics & Questions
Melatonin Preventing Estrogen-Mediated Gene Transcription In Vitro
JavaScript is disabled. For a better experience, please enable JavaScript in your browser before proceeding.
You are using an out of date browser. It may not display this or other websites correctly.
You should upgrade or use an
alternative browser
.
Reply to thread
Message
<blockquote data-quote="AhmetAtay" data-source="post: 235564" data-attributes="member: 44307"><p>This study (<a href="https://pubmed.ncbi.nlm.nih.gov/10224229/" target="_blank">Melatonin blocks the activation of estrogen receptor for DNA binding - PubMed</a>) seems to show that melatonin hinders the action of estrogen-bound estrogen receptors.</p><p></p><p>"The present study shows that <strong>melatonin prevents, within the first cell cycle, the estradiol-induced growth of synchronized MCF7 breast cancer cells</strong>. By using nuclear extracts of these cells, we first examined the binding of estradiol-estrogen receptor complexes to estrogen-responsive elements and <strong>found that the addition of estradiol to whole cells activates the binding of the estrogen receptor to DNA whereas melatonin blocks this interaction</strong>. By contrast, <strong>melatonin neither affects the binding of estradiol to its receptor nor the receptor nuclear localization</strong>. Moreover, <strong>we also show that addition of estradiol to nuclear extracts stimulates the binding of estrogen receptor to DNA, but this activation is also prevented by melatonin."</strong></p><p></p><p>In other words:</p><p></p><p>1) Melatonin does not prevent estradiol from binding to the estrogen receptor</p><p>2) Melatonin DOES, HOWEVER, interfere with the signaling mechanism mediated by the estrogen receptor which affects gene transcription.</p><p></p><p>This is analogous to how DHT derivatives like masteron appear to exert uniquely anti-estrogenic effects. They do this by not only suppressing estradiol production, but also by <strong>inhibiting estrogen-mediated gene transcription</strong></p><p></p><p>Let's get some thoughts on this!</p></blockquote><p></p>
[QUOTE="AhmetAtay, post: 235564, member: 44307"] This study ([URL="https://pubmed.ncbi.nlm.nih.gov/10224229/"]Melatonin blocks the activation of estrogen receptor for DNA binding - PubMed[/URL]) seems to show that melatonin hinders the action of estrogen-bound estrogen receptors. "The present study shows that [B]melatonin prevents, within the first cell cycle, the estradiol-induced growth of synchronized MCF7 breast cancer cells[/B]. By using nuclear extracts of these cells, we first examined the binding of estradiol-estrogen receptor complexes to estrogen-responsive elements and [B]found that the addition of estradiol to whole cells activates the binding of the estrogen receptor to DNA whereas melatonin blocks this interaction[/B]. By contrast, [B]melatonin neither affects the binding of estradiol to its receptor nor the receptor nuclear localization[/B]. Moreover, [B]we also show that addition of estradiol to nuclear extracts stimulates the binding of estrogen receptor to DNA, but this activation is also prevented by melatonin."[/B] In other words: 1) Melatonin does not prevent estradiol from binding to the estrogen receptor 2) Melatonin DOES, HOWEVER, interfere with the signaling mechanism mediated by the estrogen receptor which affects gene transcription. This is analogous to how DHT derivatives like masteron appear to exert uniquely anti-estrogenic effects. They do this by not only suppressing estradiol production, but also by [B]inhibiting estrogen-mediated gene transcription[/B] Let's get some thoughts on this! [/QUOTE]
Insert quotes…
Verification
Post reply
Share this page
Facebook
Twitter
Reddit
Pinterest
Tumblr
WhatsApp
Email
Share
Link
Sponsors
Forums
Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Basics & Questions
Melatonin Preventing Estrogen-Mediated Gene Transcription In Vitro
This site uses cookies to help personalise content, tailor your experience and to keep you logged in if you register.
By continuing to use this site, you are consenting to our use of cookies.
Accept
Learn more…
Top