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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Basics & Questions
Mass spectrometry the same as LC-MS?
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<blockquote data-quote="madman" data-source="post: 269553" data-attributes="member: 13851"><p>Yes this is the gold standard testing method for TT.</p><p></p><p>Depending on the lab some will just state MS.</p><p></p><p>Look over the methodology as it is always coupled with liquid or gas chromatography (LC or GC).</p><p></p><p></p><p></p><p></p><p><strong><em>*<u>MS-based assays remain the gold standard</u> for the quantification of total testosterone levels.</em></strong></p><p><strong><em></em></strong></p><p><strong><em>*M</em></strong><em><strong>S involves the ionization of serum compounds and subsequent measurement of their mass-to-charge ratios or molecular weight. <u>Preanalysis extraction or chromatography (gas or liquid) prior to MS can be performed to separate hormones and proteins that could otherwise affect the accurate measurement of testosterone</u>. </strong></em></p><p><em><strong></strong></em></p><p><em><strong>*The <u>MS subtype, LC-MS/MS, which couples the liquid chromatography technique of chemical separation to the MS technique</u>, has been an increasingly adopted high-throughput and accurate testosterone assay in clinical practice and research.19</strong></em></p><p></p><p></p><p></p><p></p><p>[URL unfurl="true"]https://www.excelmale.com/forum/threads/testosterone-assays.27661/[/URL]</p><p></p><p><strong>Mass Spectrometry</strong></p><p></p><p><em><strong><u>MS-based assays remain the gold standard for the quantification of total testosterone levels</u>. Despite higher costs, MS has become increasingly more utilized in clinical practice in part due to its <u>higher sensitivity and specificity at both low and high testosterone levels compared to IAs, which have been shown to vary significantly, particularly at low testosterone levels</u>.19,20</strong> From 2012 to 2015, nearly a fivefold increase in the use of MS-based assays was reported by the College of American Pathologists.14</em></p><p><em></em></p><p><em><strong>Additional advantages of MS include the ability to <u>measure multiple steroid levels simultaneously, simple sample preparation wherein nonderivatized steroids can be analyzed directly; high recovery with improved signal-to-noise ratio; and lower interference</u>.17</strong></em></p><p><em></em></p><p><em>In contrast to IA, MS involves the ionization of serum compounds and subsequent measurement of their mass-to-charge ratios or molecular weight. <strong>Preanalysis extraction or chromatography (gas or liquid) prior to MS can be performed to separate hormones and proteins that could otherwise affect the accurate measurement of testosterone. The MS subtype, LC-MS/MS, which couples the liquid chromatography technique of chemical separation to the MS technique, has been an increasingly adopted high-throughput and accurate testosterone assay in clinical practice and research.19 <u>Although considerable interlaboratory variability exists with MS, this remains a significant improvement from the variability inherent to commercially available IAs</u>.21 The high complexity of laboratory equipment required and the relatively high expense of running MS remain the predominant obstacles to its widespread adoption.22</strong></em></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><h3>Testosterone, Total, LC/MS-MS</h3><p>[URL unfurl="true"]https://www.labcorp.com/tests/070001/testosterone-total-women-children-and-hypogonadal-males-lc-ms-ms[/URL]</p><h3>Methodology</h3><p><em><strong>Liquid chromatography/tandem mass spectrometry (LC/MS-MS)</strong></em></p><p></p><p></p><p></p><p></p><h3>Testosterone, Total, MS</h3><p>[URL unfurl="true"]https://testdirectory.questdiagnostics.com/test/test-detail/15983/testosterone-total-ms?cc=MASTER[/URL]</p><p></p><h3>Methodology</h3><p><strong><em>Chromatography/Mass Spectrometry</em></strong></p></blockquote><p></p>
[QUOTE="madman, post: 269553, member: 13851"] Yes this is the gold standard testing method for TT. Depending on the lab some will just state MS. Look over the methodology as it is always coupled with liquid or gas chromatography (LC or GC). [B][I]*[U]MS-based assays remain the gold standard[/U] for the quantification of total testosterone levels. *M[/I][/B][I][B]S involves the ionization of serum compounds and subsequent measurement of their mass-to-charge ratios or molecular weight. [U]Preanalysis extraction or chromatography (gas or liquid) prior to MS can be performed to separate hormones and proteins that could otherwise affect the accurate measurement of testosterone[/U]. *The [U]MS subtype, LC-MS/MS, which couples the liquid chromatography technique of chemical separation to the MS technique[/U], has been an increasingly adopted high-throughput and accurate testosterone assay in clinical practice and research.19[/B][/I] [URL unfurl="true"]https://www.excelmale.com/forum/threads/testosterone-assays.27661/[/URL] [B]Mass Spectrometry[/B] [I][B][U]MS-based assays remain the gold standard for the quantification of total testosterone levels[/U]. Despite higher costs, MS has become increasingly more utilized in clinical practice in part due to its [U]higher sensitivity and specificity at both low and high testosterone levels compared to IAs, which have been shown to vary significantly, particularly at low testosterone levels[/U].19,20[/B] From 2012 to 2015, nearly a fivefold increase in the use of MS-based assays was reported by the College of American Pathologists.14 [B]Additional advantages of MS include the ability to [U]measure multiple steroid levels simultaneously, simple sample preparation wherein nonderivatized steroids can be analyzed directly; high recovery with improved signal-to-noise ratio; and lower interference[/U].17[/B] In contrast to IA, MS involves the ionization of serum compounds and subsequent measurement of their mass-to-charge ratios or molecular weight. [B]Preanalysis extraction or chromatography (gas or liquid) prior to MS can be performed to separate hormones and proteins that could otherwise affect the accurate measurement of testosterone. The MS subtype, LC-MS/MS, which couples the liquid chromatography technique of chemical separation to the MS technique, has been an increasingly adopted high-throughput and accurate testosterone assay in clinical practice and research.19 [U]Although considerable interlaboratory variability exists with MS, this remains a significant improvement from the variability inherent to commercially available IAs[/U].21 The high complexity of laboratory equipment required and the relatively high expense of running MS remain the predominant obstacles to its widespread adoption.22[/B][/I] [HEADING=2]Testosterone, Total, LC/MS-MS[/HEADING] [URL unfurl="true"]https://www.labcorp.com/tests/070001/testosterone-total-women-children-and-hypogonadal-males-lc-ms-ms[/URL] [HEADING=2]Methodology[/HEADING] [I][B]Liquid chromatography/tandem mass spectrometry (LC/MS-MS)[/B][/I] [HEADING=2]Testosterone, Total, MS[/HEADING] [URL unfurl="true"]https://testdirectory.questdiagnostics.com/test/test-detail/15983/testosterone-total-ms?cc=MASTER[/URL] [HEADING=2]Methodology[/HEADING] [B][I]Chromatography/Mass Spectrometry[/I][/B] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Basics & Questions
Mass spectrometry the same as LC-MS?
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