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I understand the "test in the trough" idea.  MY reasoning for testing 24 hours after my next injection (which I just took) would be to try to find the maximum T generated before the drop off starts to occur to see if the testicles are still working as intended. (maybe this wouldn't be 24 hours after injection?  Maybe I should get the blood work done today?)   At this point I just want to verify through blood work that I can actually produce T on my own.  Even though my LH was on the lower side of normal this point is kinda still up for debate because of the chemo drugs that I had.  The only studies I could find on the subject only talk about immediate (post chemo and about 1 to 2 years later) fertility.  Yes, I have felt like crap since I wrapped up chemo but I have declined over time but my sexual function has been perfectly fine except for Libido all the years prior to last year.

I think that once the above is established that a 72 hour test would be a great idea and get a good grasp on the timing of future injections.

Do you happen to know the thread name?  Or perhaps a link to it that you are referring to.  I'm not sure I read it yet and would love to and pass it along to my Dr.

I was only able to find one study regarding the leydig desensitizing and that did involve the rats. LOL

I did find another study that shows that intra muscular injections did actually last longer.  However the same study shows that HCG has a half life of about 33 hours.