I do not take the calculated dose using SES calculator, I take less than that. 1-2 ml daily or so, and as mentioned I also apply it occasionally to age-related problem areas on the skin 1-2x weekly. When I started taking it, despite lots of other vitamins, my blood tests showed my AST ALT values at the very high end of normal or slightly above range. My doctor had some concerns although I told him it was from weight lifting. I have had this discussion before, tests done after ceasing weight lifting showed my values returned to normal mid range levels. HOWEVER, after starting the C60 my AST ALT values dropped significantly despite continued weight lifting activity. Otherwise I did not feel much different with it.