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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
LabCorp Vs Quest free testosterone lab ranges: Why are they different?
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<blockquote data-quote="madman" data-source="post: 216092" data-attributes="member: 13851"><p><strong>Free testosterone by LC/MSMS - Comparison of ultrafiltration and equilibrium dialysis (2010)</strong></p><p><strong></strong></p><p><strong></strong></p><p><strong>Introduction</strong></p><p></p><p><em>Circulating testosterone (T) exists in the blood as free (FT) and protein-bound forms. Since FT is considered to be more reflective of the physiological actions of the hormone than total T, the measurement of FT is a better parameter to evaluate the androgen status and bioavailability.</em></p><p><em></em></p><p><em>Equilibrium dialysis (ED) is the gold standard method for the separation of FT. It is a time-dependent equilibrium process, that depends on various parameters, including temperature and pH. <strong><em>T</em>he objective of the project was to compare equilibrium dialysis and ultrafiltration as sample prep methods to separate FT and T. A practical LC/MSMS method needed to be validated, with an LLOQ of 1.0 pg/mL.</strong></em></p><p><em><strong></strong></em></p><p><em><strong></strong></em></p><p><em><strong>[ATTACH=full]19272[/ATTACH]</strong></em></p><p><em><strong>[ATTACH=full]19273[/ATTACH]</strong></em></p><p></p><p></p><p></p><p><strong>Conclusions</strong></p><p><strong></strong></p><p><strong><em>ED and UF methods were validated for the determination of FT in human plasma with acceptable accuracy and precision, with an LLOQ of 1 pg/mL. It was shown that FT can be reliably measured following filtration of buffered plasma, equilibrated at 37°C for 1hr, through passivated Amicon Ultra 4 (Millipore) filters at 37°C for 1hr. The UF process can be performed under controlled conditions, is simpler and faster than ED, and was, therefore, the method of choice for the bioanalysis of FT in a Phase I study of a new formulation of testosterone.</em></strong></p></blockquote><p></p>
[QUOTE="madman, post: 216092, member: 13851"] [B]Free testosterone by LC/MSMS - Comparison of ultrafiltration and equilibrium dialysis (2010) Introduction[/B] [I]Circulating testosterone (T) exists in the blood as free (FT) and protein-bound forms. Since FT is considered to be more reflective of the physiological actions of the hormone than total T, the measurement of FT is a better parameter to evaluate the androgen status and bioavailability. Equilibrium dialysis (ED) is the gold standard method for the separation of FT. It is a time-dependent equilibrium process, that depends on various parameters, including temperature and pH. [B][I]T[/I]he objective of the project was to compare equilibrium dialysis and ultrafiltration as sample prep methods to separate FT and T. A practical LC/MSMS method needed to be validated, with an LLOQ of 1.0 pg/mL. [ATTACH type="full" alt="Screenshot (10564).png"]19272[/ATTACH] [ATTACH type="full" alt="Screenshot (10565).png"]19273[/ATTACH][/B][/I] [B]Conclusions [I]ED and UF methods were validated for the determination of FT in human plasma with acceptable accuracy and precision, with an LLOQ of 1 pg/mL. It was shown that FT can be reliably measured following filtration of buffered plasma, equilibrated at 37°C for 1hr, through passivated Amicon Ultra 4 (Millipore) filters at 37°C for 1hr. The UF process can be performed under controlled conditions, is simpler and faster than ED, and was, therefore, the method of choice for the bioanalysis of FT in a Phase I study of a new formulation of testosterone.[/I][/B] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
LabCorp Vs Quest free testosterone lab ranges: Why are they different?
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