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General Health & Fitness
Health & Wellness
Illicit use of hCG in dietary programs and to promote anabolism
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<blockquote data-quote="madman" data-source="post: 245083" data-attributes="member: 13851"><p><strong>Table 30.4 Promotion of cancer cell 24 h growth at 70% confluency by C5 extravillous cytotrophoblast hCG. All experiments carried out in quadruplicate. Sixteen T75 flask of each cancer cell line grown to approximately 70% confluent. Four flasks rejected to prevent 70% confluence imbalance, 4 flask used for 70% confluence cell count. No additive flask results (-70% count) considered as blank and subtracted from all results. In case of Jar choriocarcinoma cells, for instance, mean no additive result 100,360 cells, mean 70% confluency result 128,880 cells, blank = 128880-100360 = 28,520 cells, blank = 100%. 20ng extravillous cytotrophoblast hCG mean result 158540 cells, 158540-blank = 130020 cells, 130020/100360 x 100 = 130%. (Cont.)</strong></p><p><strong>[ATTACH=full]28872[/ATTACH]</strong></p><p><strong>[ATTACH=full]28873[/ATTACH]</strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong>Table 30.4 Promotion of cancer cell 24 h growth at 70% confluency by C5 extravillous cytotrophoblast hCG. All experiments carried out in quadruplicate. Sixteen T75 flask of each cancer cell line grown to approximately 70% confluent. Four flasks rejected to prevent 70% confluence imbalance, 4 flask used for 70% confluence cell count. No additive flask results (-70% count) considered as blank and subtracted from all results. In case of Jar choriocarcinoma cells, for instance, mean no additive result 100,360 cells, mean 70% confluency result 128,880 cells, blank = 128880-100360 = 28,520 cells, blank = 100%. 20ng extravillous cytotrophoblast hCG mean result 158540 cells, 158540-blank = 130020 cells, 130020/100360 x 100 = 130%. (Cont.)</strong></p><p><strong>[ATTACH=full]28874[/ATTACH]</strong></p><p><strong>[ATTACH=full]28875[/ATTACH]</strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong>Table 30.5 Cancer cells cultured to 70% flask confluency, then cultured 24 h with antibody B152 in quadruplicate and cells counted. B152 is monoclonal antibody B152 Values are percent change from 70% confluency. The 70% confluency column lists the percentage cells and the cell count ± standard deviation, all other columns just list percentage cells ± standard deviation.</strong></p><p><strong>[ATTACH=full]28876[/ATTACH]</strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong>FIGURE 30.1 Action of hyperglycosylated hCG, hyperglycosylated hCG free β-subunit, and hCG free β-subunit on TGFβ receptor 2.</strong></p><p><strong>[ATTACH=full]28877[/ATTACH]</strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong>FIGURE 30.2 Eight nude mice transplanted with 10,000,000 cells JEG-III human choriocarcinoma cells (A), and 8 nude mice transplanted with 10,000,000 cells CaSki cervical carcinoma (B). 2 weeks later half of each 8 treated with antibody B152 and half treated with non-specific immunoglobulin G (IgG).</strong></p><p><strong>[ATTACH=full]28878[/ATTACH]</strong></p></blockquote><p></p>
[QUOTE="madman, post: 245083, member: 13851"] [B]Table 30.4 Promotion of cancer cell 24 h growth at 70% confluency by C5 extravillous cytotrophoblast hCG. All experiments carried out in quadruplicate. Sixteen T75 flask of each cancer cell line grown to approximately 70% confluent. Four flasks rejected to prevent 70% confluence imbalance, 4 flask used for 70% confluence cell count. No additive flask results (-70% count) considered as blank and subtracted from all results. In case of Jar choriocarcinoma cells, for instance, mean no additive result 100,360 cells, mean 70% confluency result 128,880 cells, blank = 128880-100360 = 28,520 cells, blank = 100%. 20ng extravillous cytotrophoblast hCG mean result 158540 cells, 158540-blank = 130020 cells, 130020/100360 x 100 = 130%. (Cont.) [ATTACH type="full"]28872[/ATTACH] [ATTACH type="full"]28873[/ATTACH] Table 30.4 Promotion of cancer cell 24 h growth at 70% confluency by C5 extravillous cytotrophoblast hCG. All experiments carried out in quadruplicate. Sixteen T75 flask of each cancer cell line grown to approximately 70% confluent. Four flasks rejected to prevent 70% confluence imbalance, 4 flask used for 70% confluence cell count. No additive flask results (-70% count) considered as blank and subtracted from all results. In case of Jar choriocarcinoma cells, for instance, mean no additive result 100,360 cells, mean 70% confluency result 128,880 cells, blank = 128880-100360 = 28,520 cells, blank = 100%. 20ng extravillous cytotrophoblast hCG mean result 158540 cells, 158540-blank = 130020 cells, 130020/100360 x 100 = 130%. (Cont.) [ATTACH type="full"]28874[/ATTACH] [ATTACH type="full"]28875[/ATTACH] Table 30.5 Cancer cells cultured to 70% flask confluency, then cultured 24 h with antibody B152 in quadruplicate and cells counted. B152 is monoclonal antibody B152 Values are percent change from 70% confluency. The 70% confluency column lists the percentage cells and the cell count ± standard deviation, all other columns just list percentage cells ± standard deviation. [ATTACH type="full"]28876[/ATTACH] FIGURE 30.1 Action of hyperglycosylated hCG, hyperglycosylated hCG free β-subunit, and hCG free β-subunit on TGFβ receptor 2. [ATTACH type="full"]28877[/ATTACH] FIGURE 30.2 Eight nude mice transplanted with 10,000,000 cells JEG-III human choriocarcinoma cells (A), and 8 nude mice transplanted with 10,000,000 cells CaSki cervical carcinoma (B). 2 weeks later half of each 8 treated with antibody B152 and half treated with non-specific immunoglobulin G (IgG). [ATTACH type="full"]28878[/ATTACH][/B] [/QUOTE]
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General Health & Fitness
Health & Wellness
Illicit use of hCG in dietary programs and to promote anabolism
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