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300IU 3x a week vs 500IU 2x week (and even 400IU 2x a week) made a BIG difference in my sperm count (sperm count much higher using bigger single dosages despite similar weekly dosage). Probably a proxy to what is going on in terms of serum concentration. In terms of how i felt. Zero difference. If fertility is a concern for you I would entertain a higher single dosage. 500UI 3x a week gave me high thyroid levels symptoms and only small gains in fertility. HCG is similar to TSH although most research on this shows that a big dosage of HCG is needed to mess with your thyroid. Apparently was not the case for me. So I am currently using 500IU 2x week and added FSH